Brassinosteroids regulate the thylakoid membrane architecture and the photosystem II function

被引：24

作者：

Krumova, S. ^{[1
]}

Zhiponova, M. ^{[2
,3
]}

Dankov, K. ^{[1
]}

Velikova, V. ^{[4
]}

Balashev, K. ^{[5
]}

Andreeva, T. ^{[1
]}

Russinova, E. ^{[2
,3
]}

Taneva, S. ^{[1
,6
]}

机构：

[1] Bulgarian Acad Sci, Inst Biophys & Biomed Engn, BU-1113 Sofia, Bulgaria

[2] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[3] Univ Ghent VIB, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent 9, Belgium

[4] Bulgarian Acad Sci, Inst Plant Physiol & Genet, BU-1113 Sofia, Bulgaria

[5] Sofia Univ St Kliment Ohridski, Fac Chem & Pharm, Dept Phys Chem, Sofia 1164, Bulgaria

[6] Univ Basque Country, Unidad Biofis CSIC UPV EHU, Dept Bioquim & Biol Mol, E-48080 Bilbao, Spain

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2013年 / 126卷

关键词：

Brassinosteroids; Photosynthesis; Thylakoid membrane; Fluorescence spectroscopy; Photosystem II function; Oxygen yield; LIGHT-HARVESTING-COMPLEX; CIRCULAR-DICHROISM; STATE TRANSITIONS; STRESS TOLERANCE; PSBQ PROTEIN; ATOMIC-FORCE; PHOSPHORYLATION; PHOTOSYNTHESIS; FLUORESCENCE; GROWTH;

D O I：

10.1016/j.jphotobiol.2013.07.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Brassinosteroids (BRs) are plant steroid hormones known to positively affect photosynthesis. In this work we investigated the architecture and function of photosynthetic membranes in mature Arabidopsis rosettes of BR gain-of-function (overexpressing the BR receptor BR INSENSITIVE 1 (BRI1), BRI1OE) and loss-of-function (bri1-116 with inactive BRI1 receptor, and constitutive photomorphogenesis and dwarfism (cpd) deficient in BR biosynthesis) mutants. Data from atomic force microscopy, circular dichroism, fluorescence spectroscopy and polarographic determination of oxygen yields revealed major structural (enlarged thylakoids, smaller photosystem II supercomplexes) and functional (strongly inhibited oxygen evolution, reduced photosystem II quantum yield) changes in all the mutants with altered BR response compared to the wild type plants. The recorded thermal dependences showed severe thermal instability of the oxygen yields in the BR mutant plants. Our results suggest that an optimal BR level is required for the normal thylakoid structure and function. (c) 2013 Elsevier B.V. All rights reserved.

引用

页码：97 / 104

页数：8

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