Genomic sequence and analysis of the atypical temperate bacteriophage N15

被引:107
作者
Ravin, V
Ravin, N
Casjens, S
Ford, ME
Hatfull, GF
Hendrix, RW
机构
[1] Russian Acad Sci, Ctr Bioengn, Moscow 117312, Russia
[2] Univ Utah, Sch Med, Dept Oncol Sci, Salt Lake City, UT 84132 USA
[3] Univ Pittsburgh, Pittsburgh Bacteriophage Inst, Pittsburgh, PA 15260 USA
[4] Univ Pittsburgh, Dept Biol Sci, Pittsburgh, PA 15260 USA
关键词
bacteriophage; linear plasmid; prophage; telomere; evolution;
D O I
10.1006/jmbi.2000.3731
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
N15 is a temperate bacteriophage that forms stable lysogens in Escherichia coli. While its virion is morphologically very similar to phage lambda and its close relatives, it is unusual in that the prophage form replicates autonomously as a linear DNA molecule with closed hairpin telomeres. Here, we describe the genomic architecture of N15, and its global pattern of gene expression, which reveal that N15 contains several plasmid-derived genes that are expressed in N15 lysogens. The tel site, at which processing occurs to form the prophage ends is close to the center of the genome in a similar location to that occupied by the attachment site, attP, in lambda and its relatives and defines the boundary between the left and right arms. The left arm contains a long cluster of structural genes that are closely related to those of the lambda-like phages, but also includes homologs of umuD', which encodes a DNA polymerase accessory protein, and the plasmid partition genes, sopA and sopB. The right arm likewise contains a mixture of apparently phage- and plasmid-derived genes including genes encoding plasmid replication functions, a phage repressor, a transcription antitermination system, as well as phage host cell lysis genes and two putative DNA methylases. The unique structure of the N15 genome suggests that the large global population of bacteriophages may exhibit a much greater diversity of genomic architectures than was previously recognized. (C) 2000 Academic Press.
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页码:53 / 73
页数:21
相关论文
共 101 条
  • [1] PARTITION OF UNIT-COPY MINIPLASMIDS TO DAUGHTER CELLS .3. THE DNA-SEQUENCE AND FUNCTIONAL-ORGANIZATION OF THE P1-PARTITION REGION
    ABELES, AL
    FRIEDMAN, SA
    AUSTIN, SJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1985, 185 (02) : 261 - 272
  • [2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [3] ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
  • [4] BAKER J, 1991, NEW BIOL, V3, P297
  • [5] SIMPLIFICATION OF PALINDROMIC TELOMERE THEORY
    BATEMAN, AJ
    [J]. NATURE, 1975, 253 (5490) : 379 - 379
  • [6] HIGH ABUNDANCE OF VIRUSES FOUND IN AQUATIC ENVIRONMENTS
    BERGH, O
    BORSHEIM, KY
    BRATBAK, G
    HELDAL, M
    [J]. NATURE, 1989, 340 (6233) : 467 - 468
  • [7] MUREIN TRANSGLYCOSYLASE FROM PHAGE LAMBDA-LYSATE - PURIFICATION AND PROPERTIES
    BIENKOWSKASZEWCZYK, K
    TAYLOR, A
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 615 (02) : 489 - 496
  • [8] TRANSCRIPTIONAL CONTROL VIA TRANSLATIONAL REPRESSION BY C4 ANTISENSE RNA OF BACTERIOPHAGE-P1 AND BACTERIOPHAGE-P7
    BIERE, AL
    CITRON, M
    SCHUSTER, H
    [J]. GENES & DEVELOPMENT, 1992, 6 (12A) : 2409 - 2416
  • [9] Two beginnings for a single purpose: The dual-start holins in the regulation of phage lysis
    Blasi, U
    Young, R
    [J]. MOLECULAR MICROBIOLOGY, 1996, 21 (04) : 675 - 682
  • [10] The complete genome sequence of Escherichia coli K-12
    Blattner, FR
    Plunkett, G
    Bloch, CA
    Perna, NT
    Burland, V
    Riley, M
    ColladoVides, J
    Glasner, JD
    Rode, CK
    Mayhew, GF
    Gregor, J
    Davis, NW
    Kirkpatrick, HA
    Goeden, MA
    Rose, DJ
    Mau, B
    Shao, Y
    [J]. SCIENCE, 1997, 277 (5331) : 1453 - +