Expression of bone morphogenetic proteins, receptors, and tissue inhibitors in human fetal, adult, and osteoarthritic articular cartilage

被引:43
作者
Chen, AL [1 ]
Fang, C [1 ]
Liu, CJ [1 ]
Leslie, MP [1 ]
Chang, E [1 ]
Di Cesare, PE [1 ]
机构
[1] NYU, Hosp Joint Dis, Inst Orthopaed, Dept Orthopaed Surg,Musculoskeletal Res Ctr, New York, NY USA
关键词
bone morphogenetic proteins; fetal; adult; osteoarthritis; cartilage;
D O I
10.1016/j.orthres.2004.02.013
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Coordinate expression of BMPs and their receptors and inhibitors is likely necessary for physiologic BMP regulation and activity. To characterize the expression of such factors in fetal, normal adult, and end-stage osteoarthritic articular cartilage, samples from these sources were analyzed. PCR-amplified sequences (BMPs 1-11), receptors (IA, IB, II), TGF-beta(1), TGF-beta(2), inhibitors noggin and follistatin, CDMP-1, COMP, and GAPDH from cDNAs generated from extracted total RNA were resolved by gel electrophoresis. Protein levels of BMPs 3, 7, and 8 were also analyzed by SDS-PAGE and Western blotting. RT-PCR revealed that BMPs 1, 2, 4-6, and 11, BMPR-IA and II, noggin, follistatin, CDMP-1, COMP, and GAPDH mRNAs were expressed in similar fashion in both fetal and adult (normal or osteoarthritic) cartilage. BMPs 9 and 10 mRNAs were not expressed in either group. BMPs 7, 8, and BMPR-IB mRNAs were consistently expressed in fetal but not in adult cartilage. BMP-3 mRNA was expressed in fetal and normal adult, but not in osteoarthritic samples. TGF-beta(1) was expressed in both adult normal and osteoarthritic, but not fetal, samples. Similarly, Western blotting demonstrated BMPs 7 and 8 to be present in fetal but not in adult samples. BMP-3 protein was present in fetal and adult normal samples, to a lesser extent, but absent in osteoarthritic cartilage. (C) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:1188 / 1192
页数:5
相关论文
共 21 条
[1]   Reduced expression of BMP-3 due to mechanical loading -: A link between mechanical stimuli and tissue differentiation [J].
Aspenberg, P ;
Basic, N ;
Tägil, M ;
Vukicevic, S .
ACTA ORTHOPAEDICA SCANDINAVICA, 2000, 71 (06) :558-562
[2]  
Bahamonde ME, 2001, J BONE JOINT SURG AM, V83A, pS56
[3]  
Candia AF, 1997, DEVELOPMENT, V124, P4467
[4]   TYPE-I RECEPTORS SPECIFY GROWTH-INHIBITORY AND TRANSCRIPTIONAL RESPONSES TO TRANSFORMING GROWTH-FACTOR-BETA AND ACTIVIN [J].
CARCAMO, J ;
WEIS, FMB ;
VENTURA, F ;
WIESER, R ;
WRANA, JL ;
ATTISANO, L ;
MASSAGUE, J .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (06) :3810-3821
[5]  
Croteau S, 1999, ORTHOPEDICS, V22, P686
[6]   Bone morphogenetic protein-3 is a negative regulator of bone density [J].
Daluiski, A ;
Engstrand, T ;
Bahamonde, ME ;
Gamer, LW ;
Agius, E ;
Stevenson, SL ;
Cox, K ;
Rosen, V ;
Lyons, KM .
NATURE GENETICS, 2001, 27 (01) :84-88
[7]   CARTILAGE OLIGOMERIC MATRIX PROTEIN - ISOLATION AND CHARACTERIZATION FROM HUMAN ARTICULAR-CARTILAGE [J].
DICESARE, PE ;
MORGELIN, M ;
CARLSON, CS ;
PASUMARTI, S ;
PAULSSON, M .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1995, 13 (03) :422-428
[8]   EXPRESSION PATTERN OF OSTEOGENIC PROTEIN-1 (BONE MORPHOGENETIC PROTEIN-7) IN HUMAN AND MOUSE DEVELOPMENT [J].
HELDER, MN ;
OZKAYNAK, E ;
SAMPATH, KT ;
LUYTEN, FP ;
LATIN, V ;
OPPERMANN, H ;
VUKICEVIC, S .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1995, 43 (10) :1035-1044
[9]  
JOHNSON EE, 1988, CLIN ORTHOP RELAT R, P249
[10]   One-stage lengthening of femoral nonunion augmented with human bone morphogenetic protein [J].
Johnson, EE ;
Urist, MR .
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH, 1998, (347) :105-116