CENP-meta, an essential kinetochore kinesin required for the maintenance of metaphase chromosome alignment in Drosophila

被引:67
作者
Yucel, JK
Marszalek, JD
McIntosh, JR
Goldstein, LSB
Cleveland, DW
Philp, AV
机构
[1] Univ Calif San Diego, Ludwig Inst Canc Res, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA
[4] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA
[5] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA
[6] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA
关键词
kinetochore; kinesin-like protein; CENP-E; chromosome congression; spindle assembly checkpoint;
D O I
10.1083/jcb.150.1.1
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element-mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements.
引用
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页码:1 / 11
页数:11
相关论文
共 56 条
[1]  
[Anonymous], 1988, Antibodies: A Laboratory Manual
[2]   Localization of the Drosophila checkpoint control protein Bub3 to the kinetochore requires Bub1 but not Zw10 or Rod [J].
Basu, J ;
Logarinho, E ;
Herrmann, S ;
Bousbaa, H ;
Li, ZX ;
Chan, GKT ;
Yen, TJ ;
Sunkel, CE ;
Goldberg, ML .
CHROMOSOMA, 1998, 107 (6-7) :376-385
[3]   Mutations in the essential spindle checkpoint gene bub1 cause chromosome missegregation and fail to block apoptosis in Drosophila [J].
Basu, J ;
Bousbaa, H ;
Logarinho, E ;
Li, ZX ;
Williams, BC ;
Lopes, C ;
Sunkel, CE ;
Goldberg, ML .
JOURNAL OF CELL BIOLOGY, 1999, 146 (01) :13-28
[4]  
Brinkley B R, 1992, Trends Cell Biol, V2, P15, DOI 10.1016/0962-8924(92)90139-E
[5]  
Brown KD, 1996, J CELL SCI, V109, P961
[6]   CYCLIN-LIKE ACCUMULATION AND LOSS OF THE PUTATIVE KINETOCHORE MOTOR CENP-E RESULTS FROM COUPLING CONTINUOUS SYNTHESIS WITH SPECIFIC DEGRADATION AT THE END OF MITOSIS [J].
BROWN, KD ;
COULSON, RMR ;
YEN, TJ ;
CLEVELAND, DW .
JOURNAL OF CELL BIOLOGY, 1994, 125 (06) :1303-1312
[7]   FUNCTIONAL CDNA LIBRARIES FROM DROSOPHILA EMBRYOS [J].
BROWN, NH ;
KAFATOS, FC .
JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (02) :425-437
[8]   Mutations of mitotic checkpoint genes in human cancers [J].
Cahill, DP ;
Lengauer, C ;
Yu, J ;
Riggins, GJ ;
Willson, JKV ;
Markowitz, SD ;
Kinzler, KW ;
Vogelstein, B .
NATURE, 1998, 392 (6673) :300-303
[9]   Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC [J].
Chan, GKT ;
Jablonski, SA ;
Sudakin, V ;
Hittle, JC ;
Yen, TJ .
JOURNAL OF CELL BIOLOGY, 1999, 146 (05) :941-954
[10]   Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1 [J].
Chan, GKT ;
Schaar, BT ;
Yen, TJ .
JOURNAL OF CELL BIOLOGY, 1998, 143 (01) :49-63