Hydrogen peroxide increases the phagocytic function of human neutrophils by calcium mobilisation

被引：25

作者：

Bejarano, I. ^{[1
]}

Terron, M. P. ^{[1
]}

Paredes, S. D. ^{[1
]}

Barriga, C. ^{[1
]}

Rodriguez, A. B. ^{[1
]}

Pariente, J. A. ^{[1
]}

机构：

[1] Univ Extremadura, Fac Sci, Dept Physiol, E-06071 Badajoz, Spain

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2007年 / 296卷 / 1-2期

关键词：

hydrogen peroxide; phagocytic function; neutrophils; calcium;

D O I：

10.1007/s11010-006-9301-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have studied the effect of exogenous administration of hydrogen peroxide (H2O2) on phagocytic activity of human neutrophils. The treatment of cells with increasing concentrations of H2O2 evoke a significant elevation of phagocytic function assayed as phagocytic index, percentage and efficiency; and was similar to that induced by the calcium mobilising agonist formyl-methionyl-leucyl-phenylalanine (fMLP). This stimulatory effect was reduced by pre-treatment of neutrophils with catalase and abolished in neutrophils loaded with the intracellular calcium quelator dimethyl BAPTA. In the absence of extracellular calcium, treatment of cells with H2O2 resulted in a increase in [Ca2+](i) , indicating the release of calcium from intracellular stores. H2O2 abolished the typical calcium release stimulated by the physiological agonist fMLP, while depletion of agonist-sensitive calcium pools by fMLP was able to prevent H2O2-induced calcium release. We conclude that H2O2 induces calcium release from agonist-sensitive stores and consequently increase the phagocytosis process.

引用

页码：77 / 84

页数：8

共 36 条

[1] Hydrogen peroxide mediates the cell growth and transformation caused by the mitogenic oxidase Nox1 [J].