Image-based adaptive optics for two-photon microscopy

被引：280

作者：

Debarre, Delphine ^{[1
]}

Botcherby, Edward J. ^{[1
]}

Watanabe, Tomoko ^{[2
]}

Srinivas, Shankar ^{[2
]}

Booth, Martin J. ^{[1
]}

Wilson, Tony ^{[1
]}

机构：

[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

[2] Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 3QX, England

来源：

OPTICS LETTERS | 2009年 / 34卷 / 16期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1364/OL.34.002495

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that, as a result, our correction process induces little photobleaching and significantly improves the quality of images of biological samples. In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues. (C) 2009 Optical Society of America

引用

页码：2495 / 2497

页数：3

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