Living Chips for drug discovery

被引：8

作者：

Kanigan, TS ^{[1
]}

Brenan, CJ ^{[1
]}

Lafontaine, S ^{[1
]}

Sosnowski, L ^{[1
]}

Madden, P ^{[1
]}

机构：

[1] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

来源：

ADVANCES IN NUCLEIC ACID AND PROTEIN ANALYSES, MANIPULATION, AND SEQUENCING | 2000年 / 1卷

关键词：

microfabrication; microarray; drug discovery; fluorescence imaging; biochip; yeast; microEDM;

D O I：

10.1117/12.380509

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We have developed novel microarray technology for performing large numbers (up to 10(6)) of chemical and cell-based assays in parallel. This technology is particularly relevant to the high-throughput screening methods used by the pharmaceutical industry to identify potential drug candidates. In this paper we provide an overview of the system and its enabling technologies, including an economical manufacturing process for creating these microarrays, a fluorescence imaging system for detecting "hits", a fluid delivery system for loading arrays, and a method for mixing reagents.

引用

页码：172 / 180

页数：9