Activation by GDNF of a transcriptional program repressing neurite growth in dorsal root ganglia

被引：17

作者：

Linnarsson, S ^{[1
]}

Mikaels, Å ^{[1
]}

Baudet, C ^{[1
]}

Ernfors, P ^{[1
]}

机构：

[1] Karolinska Inst, Mol Neurobiol Unit, Dept Med Biophys & Biochem, S-17177 Stockholm, Sweden

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 25期

关键词：

D O I：

10.1073/pnas.251548898

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Glial cell line-derived neurotrophic factor (GDNF) promotes the survival of postnatal-but not embryonic-mouse dorsal root ganglion cells in vitro, despite the fact that its receptors are expressed at both ages. To understand this difference, we have performed an oligonucleotide microarray experiment. We found that several hundred genes were regulated between embryonic and postnatal stages, and that several important classes of genes were differentially regulated by GDNF treatment, including genes related to translation and to phenotypic specification and maturation. Interestingly, a set of genes related to cell adhesion, cytoskeleton and cellular morphology were consistently downregulated by GDNF, suggesting a previously uncharacterized role for GDNF in repressing neurite growth and/or branching. This nuclear program initiated by GDNF was functionally confirmed in cultures of embryonic wild-type neurons sustained with nerve growth factor and in bax(-/-) neurons that survive in the absence of trophic support.

引用

页码：14681 / 14686

页数：6

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