Isolation and analysis of amplified cDNA fragments during detection of unknown polymorphisms with temperature modulated heteroduplex chromatography

被引：7

作者：

Kuklin, A ^{[1
]}

Munson, K ^{[1
]}

Taylor, P ^{[1
]}

Gjerde, D ^{[1
]}

机构：

[1] Transgen Inc, San Jose, CA 95131 USA

来源：

MOLECULAR BIOTECHNOLOGY | 1999年 / 11卷 / 03期

关键词：

DHPLC; TMHC; single-nucleotide polymorphism; short tandem repeat; genotyping;

D O I：

10.1007/BF02788684

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for isolation of polymerase chain reaction (PCR) fragments from an automated system for DNA fragment analysis is described. The technique is used for screening of unknown mutations or polymorphisms. Fragments are isolated during the analysis from the system by an automated unit for high-throughput projects or manually when working with a few samples.

引用

页码：257 / 261

页数：5

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