Comparison of genetic and cytogenetic maps of hexaploid wheat (Triticum aestivum L.) using SSR and DArT markers

被引：70

作者：

Francki, Michael G. ^{[1
,2
,3
]}

Walker, Esther ^{[2
,3
]}

Crawford, Allison C. ^{[1
,2
,3
]}

Broughton, Sue ^{[2
]}

Ohm, Herbert W. ^{[4
]}

Barclay, Iain ^{[2
]}

Wilson, Robin E. ^{[2
]}

McLean, Robyn ^{[2
]}

机构：

[1] Value Added Wheat Cooperat Res Ctr, N Ryde, NSW 2113, Australia

[2] Dept Agr & Food Western Australia, S Perth, WA 6151, Australia

[3] Murdoch Univ, State Agr Biotechnol Ctr, Perth, WA 6150, Australia

[4] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA

来源：

MOLECULAR GENETICS AND GENOMICS | 2009年 / 281卷 / 02期

关键词：

Doubled haploid; Methylation; Recombinant inbred line; Segregation distortion; Marker clustering; HOMOEOLOGOUS GROUP-6 CHROMOSOMES; SEGREGATION DISTORTION LOCI; QUANTITATIVE TRAIT LOCI; ARRAYS TECHNOLOGY DART; MULTIPLEX-READY PCR; L EM THELL; BREAD WHEAT; LINKAGE MAP; PHYSICAL MAPS; MICROSATELLITE MARKERS;

D O I：

10.1007/s00438-008-0403-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A number of technologies are available to increase the abundance of DNA markers and contribute to developing high resolution genetic maps suitable for genetic analysis. The aim of this study was to expand the number of Diversity Array Technology (DArT) markers on the wheat array that can be mapped in the wheat genome, and to determine their chromosomal location with respect to simple sequence repeat (SSR) markers and their position on the cytogenetic map. A total of 749 and 512 individual DArT and SSR markers, respectively, were identified on at least one of four genetic maps derived from recombinant inbred line (RIL) or doubled haploid (DH) populations. A number of clustered DArT markers were observed in each genetic map, in which 20-34% of markers were redundant. Segregation distortion of DArT and SSR markers was also observed in each mapping population. Only 14% of markers on the Version 2.0 wheat array were assigned to chromosomal bins by deletion mapping using aneuploid lines. In this regard, methylation effects need to be considered when applying DArT marker in genetic mapping. However, deletion mapping of DArT markers provides a reference to align genetic and cytogenetic maps and estimate the coverage of DNA markers across the wheat genome.

引用

页码：181 / 191

页数：11

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