High prevalence of Enterocytozoon bieneusi in swine with four genotypes that differ from those identified in humans

被引:110
作者
Breitenmoser, AC
Mathis, A
Bürgi, E
Weber, R
Deplazes, P
机构
[1] Univ Zurich, Inst Parasitol, CH-8057 Zurich, Switzerland
[2] Univ Zurich, Dept Vet Internal Med, CH-8057 Zurich, Switzerland
[3] Univ Zurich Hosp, Dept Internal Med, Div Infect Dis & Hosp Epidemiol, CH-8091 Zurich, Switzerland
关键词
Enterocytozoon bieneusi; prevalence; swine; genotypes; zoonosis;
D O I
10.1017/S0031182099004229
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The microsporidial species Enterocytozoon bieneusi is found among immunocompromised, particularly HIV-infected, patients with chronic diarrhoea, and rarely also among immunocompetent persons with self-limited diarrhoea. Only recently, E. bieneusi was detected in 4 pigs in Switzerland raising the question of a potential zoonotic nature of this parasite. We examined faecal samples of 109 pigs, 24 cows, horses and red foxes each for the presence of E. bieneusi by PCR and compared these isolates with isolates obtained from stool samples of 13 HIV-infected patients living in Switzerland. In animals, E. bieneusi was only identified in pigs with a prevalence of 35 %. Analysis of the rDNA internal transcribed spacer (ITS) sequence allowed the classification of E. bieneusi from 28 pigs into 4 distinct genotypes which grouped very closely (identity 96.3-98.8 %) together with 2 of the 3 human-derived E. bieneusi genotypes. Hence, E. bieneusi seems to be a common parasite in swine, but no genotypes were identified that were found in humans. Nevertheless, swine might serve as a new animal model for enterocytozoonosis.
引用
收藏
页码:447 / 453
页数:7
相关论文
共 30 条
[1]   Rat model for the human intestinal microsporidian Enterocytozoon bieneusi [J].
Accoceberry, I ;
Carriere, J ;
Thellier, M ;
Biligui, S ;
Danis, M ;
Datry, A .
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1997, 44 (06) :83S-83S
[2]   Rabbit model for human intestinal microsporidia [J].
Accoceberry, I ;
Greiner, P ;
Thellier, M ;
Achbarou, A ;
Biligui, S ;
Danis, M ;
Datry, A .
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1997, 44 (06) :82S-82S
[3]  
BOCH J, 1992, VETERINARMEDIZINISCH
[4]   A RAPID AND VERSATILE METHOD TO SYNTHESIZE INTERNAL STANDARDS FOR COMPETITIVE PCR [J].
CELI, FS ;
ZENILMAN, ME ;
SHULDINER, AR .
NUCLEIC ACIDS RESEARCH, 1993, 21 (04) :1047-1047
[5]   Examination of the prevalence and seasonal variation of intestinal microsporidiosis in the stools of persons with chronic diarrhea and human immunodeficiency virus infection [J].
Conteas, CN ;
Berlin, OGW ;
Lariviere, MJ ;
Pandhumas, SS ;
Speck, CE ;
Porschen, R ;
Nakaya, T .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1998, 58 (05) :559-561
[6]   Identification of Enterocytozoon bieneusi spores in respiratory samples from an AIDS patient with a 2-year history of intestinal microsporidiosis [J].
delAguila, C ;
LopezVelez, R ;
Fenoy, S ;
Turrientes, C ;
Cobo, J ;
Navajas, R ;
Visvesvara, GS ;
Croppo, GP ;
DaSilva, AJ ;
Pieniazek, NJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (07) :1862-1866
[7]   Molecular epidemiology of Encephalitozoon cuniculi and first detection of Enterocytozoon bieneusi in faecal samples of pigs [J].
Deplazes, P ;
Mathis, A ;
Muller, C ;
Weber, R .
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1996, 43 (05) :S93-S93
[8]  
DESPORTES I, 1985, J PROTOZOOL, V32, P250
[9]   IDENTIFICATION AND CHARACTERIZATION OF 3 ENCEPHALITOZOON-CUNICULI STRAINS [J].
DIDIER, ES ;
VOSSBRINCK, CR ;
BAKER, MD ;
ROGERS, LB ;
BERTUCCI, DC ;
SHADDUCK, JA .
PARASITOLOGY, 1995, 111 :411-421
[10]   Ribosomal DNA sequence of Nucleospora salmonis Hedrick, Groff and Baxa, 1991 (Microsporea: Enterocytozoonidae): Implications for phylogeny and nomenclature [J].
Docker, MF ;
Kent, ML ;
Hervio, DML ;
Khattra, JS ;
Weiss, LM ;
Devlin, RH .
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1997, 44 (01) :55-60