Investigation of the chemico-physical characteristics of the active components in the chinese herb Gastrodia elata Bl. by capillary zone electrophoresis

被引：8

作者：

Wang, DX

Yang, GL ^{[1
]}

Li, BH

Li, ZW

Chen, Y

机构：

[1] Hebei Univ, Dept Chem, Baoding 071002, Peoples R China

[2] Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China

[3] Hebei Med Coll Continue Educ, Baoding 071000, Peoples R China

来源：

ANALYTICAL SCIENCES | 2002年 / 18卷 / 04期

关键词：

D O I：

10.2116/analsci.18.409

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A CE method for investigating the chemico-physical characteristics of the active components of low molecular weight in Gastrodia elata B1. is described. First, the dissociation constants of five active components were determined based on the relation between the effective mobility of the solutes and the buffer pH value. Second, an equation that describes the relation of the migration time and the molecular weight was developed and used to predict the migration order and to calculate the electroosmotic velocity. The results predicted by theory agreed well with that from experiments.

引用

页码：409 / 412

页数：4

共 24 条

[1] UV-vis spectroscopic determination of the dissociation constant of bichromate from 160 to 400 °C [J].