Mouse Dach, a homologue of Drosophila dachshund, is expressed in the developing retina, brain and limbs

被引:88
作者
Davis, RJ
Shen, WP
Heanue, TA
Mardon, G
机构
[1] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA
[2] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
[3] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA
[4] Baylor Coll Med, Dept Ophthalmol, Houston, TX 77030 USA
[5] Baylor Coll Med, Program Dev Biol, Houston, TX 77030 USA
基金
美国国家卫生研究院;
关键词
dachshund; retina; development; limb; brain;
D O I
10.1007/s004270050285
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The Drosophila genes eyeless, eyes absent sine oculis, and dachshund cooperate as key regulators of retinal cell-fate determination. Homologues of eyeless (Pax6), eyes absent (Eyal-2), and sine oculis (Six3) have been identified and are expressed in the developing vertebrate eye. We have cloned and characterized the structure and expression of mouse Dach, a homologue of Drosophila dachshund. Sequence analysis reveals the presence of two motifs, DD1 and DD2, which may be involved in the function of Dach/Dacbshund as gene regulatory factors. In addition, DD1 shares sequence similarity to N-terminal sequences of Ski and SnoN, which are involved in cellular transformation and differentiation. Mouse and human Dach/DACH were localized to chromosome 14E1 and 13q21.3-22, respectively, by fluorescence in situ hybridization. Finally, in situ hybridization analysis demonstrated that Dach is expressed in similar tissues to those observed in Drosophila, including the embryonic nervous system, sensory organs, and limbs. The finding of Dach expression in the eye completes the list of vertebrate homologues of eyeless, eyes absent, sine oculis, and dachshund which as a group may function to control cell-fate determination in the vertebrate eye.
引用
收藏
页码:526 / 536
页数:11
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