Cytochemical and Western blot analysis of the subcompartmentalization of the acrosome in rodents using soybean lectin

被引:7
作者
Martínez-Menárguez, JA [1 ]
Abascal, I [1 ]
Aviles, M [1 ]
Castells, MT [1 ]
Ballesta, J [1 ]
机构
[1] Univ Murcia, Sch Med, Dept Cell Biol, Sect Histol & Gen Embryol, E-30071 Murcia, Spain
来源
HISTOCHEMICAL JOURNAL | 1999年 / 31卷 / 01期
关键词
D O I
10.1023/A:1003561230689
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In the present study, the formation and development of the acrosome during spermiogenesis in four different rodent species (rat, mouse, hamster and guinea pig) was compared by means of cytochemical and blotting techniques using a lectin from soybean (SBA). This lectin recognizes specifically the acrosome of the four species at all steps of formation. At the ultrastructural level, SBA-binding pattern was similar in the acrosome of the rat, mouse and hamster. SBA preferentially labelled the electron-lucent area of the acrosome in early spermatids (Golgi and cap phases) and the outer region of the acrosome in mature spermatids (acrosome and maturation phase). The lectin binding pattern was more complex in the guinea pig acrosome. Three different subdomains can be established in the early acrosome of the guinea pig. The lectin bound the three subdomains but mainly a thin fold which spreads over the nucleus during the cap phase. In the acrosome phase, SBA strongly reacted with the principal segment. In contrast, no reactivity was observed in most of this segment in maturation phase spermatids. In this phase, SBA bound preferentially a thin area covering the dorsal region of the apical segment. Lectin blots of detergent-extracted testes indicated that SBA only recognizes proteins of high molecular weight (> 100 kD) in the four species studied. The results obtained in the present study suggest that the development of acrosomal subdomains is very similar in the mouse, rat and hamster but shows a more complex pattern in the guinea pig.
引用
收藏
页码:29 / 37
页数:9
相关论文
共 26 条
[1]   ACROSOME BIOGENESIS BEGINS DURING MEIOSIS - EVIDENCE FROM THE SYNTHESIS AND DISTRIBUTION OF AN ACROSOMAL GLYCOPROTEIN, ACROGRANIN, DURING GUINEA-PIG SPERMATOGENESIS [J].
ANAKWE, OO ;
GERTON, GL .
BIOLOGY OF REPRODUCTION, 1990, 42 (02) :317-328
[2]  
BALLESTA J, 1991, EUR J BASIC APPL HIS, V35, P107
[3]   LOCALIZATION OF BOAR SPERM PROACROSIN DURING SPERMATOGENESIS AND DURING SPERM MATURATION IN THE EPIDIDYMIS [J].
BOZZOLA, JJ ;
POLAKOSKI, K ;
HAAS, N ;
RUSSELL, LD ;
CAMPBELL, P ;
PETERSON, RN .
AMERICAN JOURNAL OF ANATOMY, 1991, 192 (02) :129-141
[4]  
Foster JA, 1996, MOL REPROD DEV, V44, P221, DOI 10.1002/(SICI)1098-2795(199606)44:2&lt
[5]  
221::AID-MRD11&gt
[6]  
3.0.CO
[7]  
2-5
[8]   PURIFICATION AND CHARACTERIZATION OF THE PRIMARY ACROSOMAL AUTO-ANTIGEN OF GUINEA-PIG EPIDIDYMAL SPERMATOZOA [J].
HARDY, DM ;
HUANG, TTF ;
DRISCOLL, WJ ;
TUNG, KSK ;
WILD, GC .
BIOLOGY OF REPRODUCTION, 1988, 38 (02) :423-437
[9]   A MECHANISM FOR DIFFERENTIAL RELEASE OF ACROSOMAL ENZYMES DURING THE ACROSOME REACTION [J].
HARDY, DM ;
ODA, MN ;
FRIEND, DS ;
HUANG, TTF .
BIOCHEMICAL JOURNAL, 1991, 275 :759-766
[10]   LOCALIZATION OF SPERM ANTIGEN SP-10 DURING THE 6 STAGES OF THE CYCLE OF THE SEMINIFEROUS EPITHELIUM IN MAN [J].
KURTH, BE ;
KLOTZ, K ;
FLICKINGER, CJ ;
HERR, JC .
BIOLOGY OF REPRODUCTION, 1991, 44 (05) :814-821