Evaluation of a real-time PCR kit for detecting Escherichia coli O157 in bovine fecal samples

被引：23

作者：

Bono, JL ^{[1
]}

Keen, JE ^{[1
]}

Miller, LC ^{[1
]}

Fox, JM ^{[1
]}

Chitko-McKown, CG ^{[1
]}

Heaton, MP ^{[1
]}

Laegreid, WW ^{[1
]}

机构：

[1] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 03期

关键词：

D O I：

10.1128/AEM.70.3.1855-1857.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g(-1) in artificially inoculated bovine feces following enrichment.

引用

页码：1855 / 1857

页数：3

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