Combinatorial Gata2 and Sca1 expression defines hematopoietic stem cells in the bone marrow niche

被引：83

作者：

Suzuki, N

Ohneda, O

Minegishi, N

Nishikawa, M

Ohta, T

Takahashi, S

Engel, JD

Yamamoto, M

机构：

[1] Univ Michigan, Sch Med, Dept Cell & Dev Biol, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Sch Med, Ctr Organogenesis, Ann Arbor, MI 48109 USA

[3] ERATO, JST, Environ Responses Project, Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki 3058577, Japan

[4] Univ Tsukuba, Grad Sch Comprehens Human Sci, Tsukuba, Ibaraki 3058577, Japan

[5] Kirir Brewery Pharmaceut Res Lab, Takasaki, Gumma 3701295, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2006年 / 103卷 / 07期

关键词：

GATA-2; GFP knock-in mouse; imaging;

D O I：

10.1073/pnas.0508928103

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The interaction between stem cells and their supportive microenvironment is critical for their maintenance, function, and survival. Whereas hematopoietic stem cells (HSCs) are among the best characterized of tissue stem cells, their precise site of residence (referred to as the niche) in the adult bone marrow has not been precisely defined. In this study, we found that a Gata2 promoter directs activity in all HSCs. We show that HSCs can be isolated efficiently from bone marrow cells by following Gata2-directed GFP fluorescence, and that they can also be monitored in vivo. Each individual GFP-positive cell lay in a G(0)/G(1) cell cycle state, in intimate contact with osteoblasts beside the endosteum, at the edge of the bone marrow. We conclude that the HSC niche is composed of solitary cells and that adult bone marrow HSC are not clustered.

引用

页码：2202 / 2207

页数：6

共 32 条

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