Activity-based probes as a tool for functional proteomic analysis of proteases

被引：178

作者：

Fonovic, Marko ^{[1
]}

Bogyo, Matthew ^{[2
]}

机构：

[1] Jozef Stefan Inst, Dept Biochem Mol & Struct Biol, SI-1000 Ljubljana, Slovenia

[2] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA

来源：

EXPERT REVIEW OF PROTEOMICS | 2008年 / 5卷 / 05期

关键词：

activity-based probe; protease; proteolysis; proteomics;

D O I：

10.1586/14789450.5.5.721

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Traditional proteomics methodology allows global analysis of protein abundance but does not provide information on the regulation of protein activity. Proteases, in particular, are known for their multilayered post-translational activity regulation that can lead to a significant difference between protease abundance levels and their enzyme activity. To address these issues, the field of activity-based proteomics has been established in order to characterize protein activity and monitor the functional regulation of enzymes in complex proteomes. In this review, we present structural features of activity-based probes for proteases and discuss their applications in proteomic profiling of various catalytic classes of proteases.

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收藏

页码：721 / 730

页数：10

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