Cloning and characterization of an epoxide hydrolase-encoding gene from Rhodotorula glutinis

被引：41

作者：

Visser, H ^{[1
]}

Vreugdenhil, S ^{[1
]}

de Bont, JAM ^{[1
]}

Verdoes, JC ^{[1
]}

机构：

[1] Wageningen Univ Agr, Dept Food Technol & Nutr Sci, Div Ind Microbiol, NL-6700 EV Wageningen, Netherlands

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2000年 / 53卷 / 04期

关键词：

D O I：

10.1007/s002530051635

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We cloned and characterized the epoxide hydrolase gene, EPH1, from Rhodotorula glutinis. The EPH1 open reading frame of 1230 bp was interrupted by nine introns and encoded a polypeptide of 409 amino acids with a calculated molecular mass of 46.3 kDa. The amino acid sequence was similar to that of microsomal epoxide hydrolase, which suggests that the epoxide hydrolase of R. glutinis also belongs to the alp hydrolase fold family. EPH1 cDNA was expressed in Escherichia coli and resting cells showed a specific activity of 200 nmol min(-1) (mg protein)(-1) towards 1,2-epoxyhexane.

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页码：415 / 419

页数：5

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