LookSeq: A browser-based viewer for deep sequencing data

被引：41

作者：

Manske, Heinrich Magnus ^{[1
]}

Kwiatkowski, Dominic P. ^{[1
]}

机构：

[1] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

来源：

GENOME RESEARCH | 2009年 / 19卷 / 11期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

HUMAN GENOME; ALIGNMENT; PROGRAM;

D O I：

10.1101/gr.093443.109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequencing a genome to great depth can be highly informative about heterogeneity within an individual or a population. Here we address the problem of how to visualize the multiple layers of information contained in deep sequencing data. We propose an interactive AJAX-based web viewer for browsing large data sets of aligned sequence reads. By enabling seamless browsing and fast zooming, the LookSeq program assists the user to assimilate information at different levels of resolution, from an overview of a genomic region to fine details such as heterogeneity within the sample. A specific problem, particularly if the sample is heterogeneous, is how to depict information about structural variation. LookSeq provides a simple graphical representation of paired sequence reads that is more revealing about potential insertions and deletions than are conventional methods.

引用

页码：2125 / 2132

页数：8

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