Fixation-dependent organization of core histones following DNA fluorescent in situ hybridization

被引：18

作者：

Hendzel, MJ ^{[1
]}

BazettJones, DP ^{[1
]}

机构：

[1] UNIV CALGARY,FAC MED,DEPT ANAT & MED BIOCHEM,CALGARY,AB T2N 4N1,CANADA

来源：

CHROMOSOMA | 1997年 / 106卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1007/s004120050231

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have evaluated the effects of different DNA denaturation protocols commonly used in DNA fluorescent in situ hybridization (FISH) experiments on chromatin structure using indirect immunofluorescence. The use of antibodies to acetylated histones H3 and H4 demonstrates that the different procedures differ considerably in their extent of histone displacement. Procedures involving paraformaldehyde fixation were found to be compatible with the structural preservation of acetylated chromatin organization by indirect immunofluorescence. These results provide a basis for interpreting DNA FISH experiments aimed at determining chromatin organization of individual loci.

引用

页码：114 / 123

页数：10

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