In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus

被引：69

作者：

Diaz, Ivan ^{[1
]}

Pujols, Joan ^{[1
]}

Ganges, Llilianne ^{[1
,3
]}

Gimeno, Mariona ^{[1
,2
]}

Darwich, Laila ^{[1
,2
]}

Domingo, Mariano ^{[1
,2
]}

Mateu, Enric ^{[1
,2
]}

机构：

[1] UAB, IRTA, CReSA, Barcelona 08193, Spain

[2] Univ Autonoma Barcelona, Dept Sanitat & Anat Anim, E-08193 Barcelona, Spain

[3] Inst Nacl Invest & Tecnol Agr & Alimentaria, Madrid, Spain

来源：

VACCINE | 2009年 / 27卷 / 41期

关键词：

Porcine reproductive and respiratory syndrome virus; T-cell epitopes; Bioinformatics; LELYSTAD-VIRUS; NEUTRALIZING ANTIBODIES; PHYLOGENETIC ANALYSIS; GENETIC DIVERSITY; IMMUNE-RESPONSES; PASSIVE TRANSFER; EASTERN-EUROPE; DNA VACCINE; IFN-GAMMA; PROTECTION;

D O I：

10.1016/j.vaccine.2009.07.029

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

T-cell epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) glycoproteins 4 (GP4), 5 (GP5) and nucleocapsid (N) were predicted using bioinformatics and later tested by IFN-gamma ELISPOT in pigs immunized with either a modified live vaccine (MLV) or DNA (open reading frames 4, 5 or 7). For MLV-vaccinated pigs, immunodominant epitopes were found in N but T-epitopes were also found in GP4 and GP5. For DNA-immunized pigs, some peptides were differently recognized. Using a large set of PRRSV sequences it was shown that N contains a conserved epitope and that for GP5, the genotype-I counterparts of previously reported epitopes of genotype-II strains were also immunogenic. (c) 2009 Elsevier Ltd. All rights reserved.

引用

页码：5603 / 5611

页数：9

共 42 条

[1] T-cell epitope mapping using the ELISPOT approach [J].