Modified pyrimidines specifically bind the purine riboswitch

被引：69

作者：

Gilbert, Sunny D. ^{[1
]}

Mediatore, Sarah J. ^{[1
]}

Batey, Robert T. ^{[1
]}

机构：

[1] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 44期

关键词：

D O I：

10.1021/ja063645t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The purine riboswitch is a genetic regulatory element found in the 5′-untranslated regions of Gram-positive bacteria that regulates expression of the mRNA specifically in response to either guanine or adenine. We report that the adenine-responsive RNA element is also capable of specifically recognizing pyrimidine compounds bearing modifications at the 6- or 5,6-positions in a fashion similar to that of purine compounds. Using isothermal titration calorimetry and X-ray crystallography, the binding of these compounds is characterized. Copyright © 2006 American Chemical Society.

引用

页码：14214 / 14215

页数：2

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