Inhibitory effect of phorbol 12,13 dibutyrate on carbachol-activated nonselective cationic current in guinea-pig gastric myocytes

被引:17
作者
Ahn, SC [1 ]
Kim, SJ [1 ]
So, I [1 ]
Kim, KW [1 ]
机构
[1] SEOUL NATL UNIV,COLL MED,DEPT PHYSIOL & BIOPHYS,CHONGNO GU,SEOUL 110799,SOUTH KOREA
来源
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1997年 / 434卷 / 04期
关键词
carbachol; nonselective cationic current; protein kinase C; smooth muscle;
D O I
10.1007/s004240050429
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The effect of protein kinase C (PKC) on carbachol (CCh)-activated nonselective cationic current (I-CCh) Was investigated in guinea-pig gastric myocytes using a PKC activator, phorbol 12, 13 dibutyrate (PDBu). Pretreatment with 1 mu M PDBu suppressed I-CCh by 96.5 +/- 2.9% (n = 14) in a reversible manner in nystatin-perforated mode. In the presence of 1 mu M chelerythrine , a PKC inhibitor, inhibition of I-CCh by PDBu was not seen. In whole-cell mode, the inhibition of I-CCh by PDBu was dependent on intracellular MgATP. In the presence of MgATP in the pipette, PDBu decreased I-CCh by 98.8 +/- 1.2% (n = 5) as was observed in nystatin-perforated mode. However, PDBu had little effect on I-CCh in the absence of MgATP in the pipette; the extent of inhibition was 12.7 +/- 4.3% (n = 8). PDBu also suppressed the generation of cationic current induced by intracellularly perfused GTP[gamma S]. In the PDBu-pretreated group (n = 9) and PDBu-untreated control group (n = 6), GTP[gamma S]-induced currents were 6.7 +/- 2.4 pA and 236 +/- 23 pA, respectively. These results suggest that PKC modulates I-CCh at postreceptor sites via protein phosphorylation.
引用
收藏
页码:505 / 507
页数:3
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