Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction

被引:58
作者
Chen, Qing-Rong
Vansant, Gordon
Oades, Kahuku
Pickering, Maria
Wei, Jun S.
Song, Young K.
Monforte, Joseph
Khan, Javed
机构
[1] NCI, Oncogenom Sect, Pediat Oncol Branch, Ctr Adv Technol, Gaithersburg, MD 20877 USA
[2] NCI, SAIC Frederick Inc, Adv Biomed Comp Ctr, Frederick, MD 21701 USA
[3] Althea Technol, San Diego, CA USA
关键词
D O I
10.2353/jmoldx.2007.060111
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The small round blue cell tumors of childhood, which include neuroblastoma, rhabdomyosarcoma, non Hodgkin's lymphoma, and the Ewing's family of tumors, are so called because of their similar appearance on routine histology. Using cDNA microarray gene expression profiles and artificial neural networks (ANNs), we previously identified 93 genes capable of diagnosing these cancers. Using a subset of these, together with some additional genes (total 39), we developed a multiplex polymerase chain reaction (PCR) assay to diagnose these cancer types. Blinded testing of 96 new samples (26 Ewing's family of tumors, 29 rhabdomyosarcomas, 24 neuroblastomas, and 17 lymphomas) using ANNs in a complete leave-one-out analysis demonstrated that all except one sample were accurately diagnosed as their respective category. Moreover, using an ANN-based gene minimization strategy in a separate analysis, we found that the top 31 genes could correctly diagnose an 96 tumors. Our results suggest that this molecular test based on a multiplex PCR reaction may assist the physician in the rapid confirmation of the diagnosis of these cancers.
引用
收藏
页码:80 / 88
页数:9
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