One-step homogeneous detection of DNA hybridization with gold nanoparticle probes by using a linear light-scattering technique

被引:152
作者
Du, Bao-An [1 ]
Li, Zheng-Ping [1 ]
Liu, Cheng-Hui [1 ]
机构
[1] Hebei Univ, Coll Chem & Environm Sci, Baoding 071002, Hebei Province, Peoples R China
关键词
base-pair mismatches; DNA recognition; gold nanoparticles; light scattering; nucleoticles;
D O I
10.1002/anie.200603331
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
(Figure Presented) Matchmaker, matchmaker: A gold-nanoparticle-based light-scattering assay has been developed for sensitive detection of DNA hybridization. The assay relies on the enhancement of light scattering that originates from the aggregation of oligonucleotide-functionalized gold nanoparticles directed by the target DNA. The assay also exhibits a high degree of discrimination between perfectly matched target DNA and targets with single base-pair mismatches. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码:8022 / 8025
页数:4
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