Systemic Induction and Role of Mitochondrial Alternative Oxidase and Nitric Oxide in a Compatible Tomato-Tobacco mosaic virus Interaction

被引：78

作者：

Fu, Li-Jun ^{[1
]}

Shi, Kai ^{[1
]}

Gu, Min ^{[1
]}

Zhou, Yan-Hong ^{[1
]}

Dong, De-Kun ^{[2
]}

Liang, Wu-Sheng ^{[3
]}

Song, Feng-Ming ^{[3
]}

Yu, Jing-Quan ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Hort, Hangzhou 310003, Zhejiang, Peoples R China

[2] Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou, Zhejiang, Peoples R China

[3] Zhejiang Univ, Inst Biotechnol, Dept Plant Protect, Hangzhou 310003, Zhejiang, Peoples R China

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2010年 / 23卷 / 01期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

ACID-INDUCED RESISTANCE; SALICYLIC-ACID; ARABIDOPSIS-THALIANA; TRANSGENIC TOBACCO; SIGNALING PATHWAYS; ELECTRON-TRANSPORT; DOWN-REGULATION; NADPH OXIDASE; UP-REGULATION; EXPRESSION;

D O I：

10.1094/MPMI-23-1-0039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of mitochondrial alternative oxidase (AOX) and the relationship between AOX and nitric oxide (NO) in virus-induced systemic defense to Tobacco mosaic virus (TMV) were investigated in susceptible tomato (Solanum lycopersicum) plants. TMV inoculation to the lower leaves induced a rapid NO synthesis and AOX activation in upper uninoculated leaves as early as 0.5 day postinoculation. Application of exogenous potassium cyanide (KCN, a cytochrome pathway inhibitor) at nonlethal concentrations and NO donor diethylamine NONOate (DEA/NO) to the upper uninoculated leaves greatly induced accumulation of AOX transcript, reduced TMV viral RNA accumulation, and increased the leaf photochemical quantum yield at photosystem II. Pretreatment with NO scavenger almost completely blocked TMV-induced AOX induction and substantially increased TMV susceptibility. Salicylhydroxamic acid (SHAM, an AOX inhibitor) pretreatment reduced the DEA/NO-induced cyanide-resistant respiration and partially compromised induced resistance to TMV. Conversely, KCN and SHAM pretreatment had very little effect on generation of NO, and pretreatment with NO scavenger did not affect KCN-induced AOX induction and TMV resistance. These results suggest that TMV-induced NO generation acts upstream and mediates AOX induction which, in turn, induces mitochondrial alternative electron transport and triggers systemic basal defense against the viral pathogen.

引用

页码：39 / 48

页数：10

共 64 条

[1] An early tobacco mosaic virus-induced oxidative burst in tobacco indicates extracellular perception of the virus coat protein [J].

Allan, AC ;

Lapidot, M ;

Culver, JN ;

Fluhr, R .

PLANT PHYSIOLOGY, 2001, 126 (01) :97-108

[2] The role of the mitochondrion in plant responses to biotic stress [J].

Amirsadeghi, Sasan ;

Robson, Christine A. ;

Vanlerberghe, Greg C. .

PHYSIOLOGIA PLANTARUM, 2007, 129 (01) :253-266

[3] AOX - a functional marker for efficient cell reprogramming under stress? [J].

Arnholdt-Schmitt, Birgit ;

Costa, Jose H. ;

de Melo, Dirce Fernandes .

TRENDS IN PLANT SCIENCE, 2006, 11 (06) :281-287

[4] MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana [J].

Asai, Shuta ;

Ohta, Kohji ;

Yoshioka, Hirofumi .

PLANT CELL, 2008, 20 (05) :1390-1406

[5] Disruption of the alternative oxidase gene in Magnaporthe grisea and its impact on host infection [J].

Avila-Adame, C ;

Köller, W .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2002, 15 (05) :493-500

[6]

Chivasa S, 1997, PLANT CELL, V9, P547, DOI 10.1105/tpc.9.4.547

[7] Cyanide restores N gene-mediated resistance to tobacco mosaic virus in transgenic tobacco expressing salicylic acid hydroxylase [J].

Chivasa, S ;

Carr, JP .

PLANT CELL, 1998, 10 (09) :1489-1498

[8] Cellular and subcellular localization of endogenous nitric oxide in young and senescent pea plants [J].

Corpas, FJ ;

Barroso, JB ;

Carreras, A ;

Quirós, M ;

León, AM ;

Romero-Puertas, MC ;

Esteban, FJ ;

Valderrama, R ;

Palma, JM ;

Sandalio, LM ;

Gómez, M ;

del Río, LA .

PLANT PHYSIOLOGY, 2004, 136 (01) :2722-2733

[9] The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1 [J].

Després, C ;

Chubak, C ;

Rochon, A ;

Clark, R ;

Bethune, T ;

Desveaux, D ;

Fobert, PR .

PLANT CELL, 2003, 15 (09) :2181-2191

[10] Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose [J].

Durner, J ;

Wendehenne, D ;

Klessig, DF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (17) :10328-10333

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