Detection of Toscana virus specific immunoglobulins G and M by an enzyme-linked immunosorbent assay based on recombinant viral nucleoprotein

被引：18

作者：

Ciufolini, MG ^{[1
]}

Fiorentini, C ^{[1
]}

Di Bonito, P ^{[1
]}

Mochi, S ^{[1
]}

Giorgi, C ^{[1
]}

机构：

[1] Ist Super Sanita, Virol Lab, I-00161 Rome, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 06期

关键词：

D O I：

10.1128/JCM.37.6.2010-2012.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An enzyme-linked immunosorbent assay (ELISA) based on the recombinant Toscana virus nucleoprotein (rN) has been developed. Its sensitivity and specificity for the detection of virus-specific immunoglobulins G and M in human sera were similar to those of the ELISA that is based on an antigen extracted from infected mouse brain and that is routinely used for serodiagnosis.

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页码：2010 / 2012

页数：3

相关论文

共 18 条

[1] Toscana virus infections of the central nervous system in children: A report of 14 cases [J].

Braito, A ;

Corbisiero, R ;

Corradini, S ;

Fiorentini, C ;

Ciufolini, MG .

JOURNAL OF PEDIATRICS, 1998, 132 (01) :144-148

[2] Evidence of Toscana virus infections without central nervous system involvement: A serological study [J].

Braito, A ;

Corbisiero, R ;

Corradini, S ;

Marchi, B ;

Sancasciani, N ;

Fiorentini, C ;

Ciufolini, MG .

EUROPEAN JOURNAL OF EPIDEMIOLOGY, 1997, 13 (07) :761-764

[3]

CALISHER CH, 1987, LANCET, V1, P165

[4] TECHNIQUES FOR HEMAGGLUTINATION AND HEMAGGLUTINATION-INHIBITION WITH ARTHROPOD-BORNE VIRUSES [J].

CLARKE, DH ;

CASALS, J .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1958, 7 (05) :561-573

[5]

DIBONITO P, UNPUB

[6]

EHRNST A, 1985, LANCET, V1, P1212

[7] SEQUENCES AND CODING STRATEGIES OF THE S-RNAS OF TOSCANA AND RIFT-VALLEY FEVER VIRUSES COMPARED TO THOSE OF PUNTA-TORO, SICILIAN SANDFLY FEVER, AND UUKUNIEMI VIRUSES [J].

GIORGI, C ;

ACCARDI, L ;

NICOLETTI, L ;

GRO, MC ;

TAKEHARA, K ;

HILDITCH, C ;

MORIKAWA, S ;

BISHOP, DHL .

VIROLOGY, 1991, 180 (02) :738-753

[8] IDENTIFICATION OF HANTAVIRUS SEROTYPES BY TESTING OF POSTINFECTION SERA IN IMMUNOFLUORESCENCE AND ENZYME-LINKED IMMUNOSORBENT ASSAYS [J].

GROEN, J ;

JORDANS, HGM ;

CLEMENT, JPG ;

ROOIJAKKERS, EJM ;

UYTDEHAAG, FGCM ;

DALRYMPLE, J ;

VANDERGROEN, G ;

OSTERHAUS, ADME .

JOURNAL OF MEDICAL VIROLOGY, 1991, 33 (01) :26-32

[9] Humoral response in Toscana virus acute neurologic disease investigated by viral-protein-specific immunoassays [J].

Magurano, F ;

Nicoletti, L .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1999, 6 (01) :55-60

[10] CENTRAL-NERVOUS-SYSTEM INVOLVEMENT DURING INFECTION BY PHLEBOVIRUS-TOSCANA OF RESIDENTS IN NATURAL FOCI IN CENTRAL ITALY (1977-1988) [J].

NICOLETTI, L ;

VERANI, P ;

CACIOLLI, S ;

CIUFOLINI, MG ;

RENZI, A ;

BARTOLOZZI, D ;

PACI, P ;

LEONCINI, F ;

PADOVANI, P ;

TRAINI, E ;

BALDERESCHI, M ;

BALDUCCI, M .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1991, 45 (04) :429-434