Characterization of the first definitive hematopoietic stem cells in the AGM and liver of the mouse embryo

被引:248
作者
Sanchez, MJ [1 ]
Holmes, A [1 ]
Miles, C [1 ]
Dzierzak, E [1 ]
机构
[1] NATL INST MED RES, LONDON NW7 1AA, ENGLAND
基金
英国医学研究理事会;
关键词
D O I
10.1016/S1074-7613(00)80267-8
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
At day 10 in mouse gestation, the intraembryonic aorta-gonads-mesonephros (AGM) region generates the first definitive hematopoietic stem cells (HSCs) of the adult blood system. By 11 days postcoitum, the liver contains such HSCs. While HSCs of the adult bone marrow and late-stage fetal liver have been extensively characterized for cell surface markers, there has been no phenotypic description of the first HSCs during embryo development. We report here the temporal cell surface phenotype of HSCs from the AGM region and early fetal liver and show that all HSCs reside in the c-kit(+) population. c-kit(+) HSCs from AGM and liver are mainly CD34(+) and in the AGM are in both Mac-1(+) and Mac-1 fractions. These results demonstrate that during mouse ontogeny the first definitive HSCs are similar in cell surface phenotype to the HSCs of adult bone marrow but that spatial localization and developmental time are critical factors in the phenotypic assessment of this functional cell population.
引用
收藏
页码:513 / 525
页数:13
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