Dexamethasone-induced insulin resistance and pancreatic adaptive response in aging rats are not modified by oral vanadyl sulfate treatment

被引:29
作者
Barbera, M [1 ]
Fierabracci, V [1 ]
Novelli, M [1 ]
Bombara, M [1 ]
Masiello, P [1 ]
Bergamini, E [1 ]
De Tata, V [1 ]
机构
[1] Univ Studi Pisa, Dipartimento Patol Sperimentale, BMIE, I-56126 Pisa, Italy
关键词
D O I
10.1530/eje.0.1450799
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: To explore the adaptive response of the endocrine pancreas in vivo and in vitro and the possible beneficial effect of the insulino-mimetic agent vanadyl sulfate (VOSO4), using glucocorticoid treatment to increase insulin resistance, in aging rats. Design and Methods: Dexamethasone (Dex) (0.13 mg/kg b.w.) was administered daily for 13 days to Sand 18-month old Sprague-Dawley rats and oral VOSO4 was given from the 5th day. Plasma glucose, insulin and free fatty acids (FFA) concentrations were measured during these treatments and the insulin secretory response of the isolated perfused pancreas was assessed at the end of the experiment. Results and Conclusions: In both young and aging rats, particularly in the latter, hyperinsulinemia and increased in vitro insulin responsiveness to glucose were observed in response to Dex treatment, concomitant with an increase in plasma FFA concentrations. Thus, in glucocorticoid-treated animals, the beta -cell adaptive response occurred in both age groups and could possibly be mediated by increased circulating FFA: however, it was insufficient to prevent hyperglycemia in 60% of aging animals. Oral VOSO4 administration failed to correct Dex-induced alterations in glucose and lipid metabolism, although it influenced in vitro beta -cell responsiveness to stimuli in aging rats.
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收藏
页码:799 / 806
页数:8
相关论文
共 43 条
[1]   INVIVO INSULIN RESISTANCE IN STREPTOZOTOCIN-DIABETIC RATS - EVIDENCE FOR REVERSAL FOLLOWING ORAL VANADATE TREATMENT [J].
BLONDEL, O ;
BAILBE, D ;
PORTHA, B .
DIABETOLOGIA, 1989, 32 (03) :185-190
[2]  
BODEN G, 1996, DIABETES, V45, P3
[3]   ORAL VANADATE DECREASES MUSCLE INSULIN RESISTANCE IN OBESE FA/FA RATS [J].
BRICHARD, SM ;
ONGEMBA, LN ;
HENQUIN, JC .
DIABETOLOGIA, 1992, 35 (06) :522-527
[4]   LONG-TERM IMPROVEMENT OF GLUCOSE-HOMEOSTASIS BY VANADATE IN OBESE HYPERINSULINEMIC FA/FA RATS [J].
BRICHARD, SM ;
POTTIER, AM ;
HENQUIN, JC .
ENDOCRINOLOGY, 1989, 125 (05) :2510-2516
[5]  
BRICHARD SM, 1991, DIABETES METAB, V17, P435
[6]   LONG-TERM IMPROVEMENT OF GLUCOSE-HOMEOSTASIS BY VANADATE TREATMENT IN DIABETIC RATS [J].
BRICHARD, SM ;
OKITOLONDA, W ;
HENQUIN, JC .
ENDOCRINOLOGY, 1988, 123 (04) :2048-2053
[7]   MARKED IMPROVEMENT OF GLUCOSE-HOMEOSTASIS IN DIABETIC OB OB MICE GIVEN ORAL VANADATE [J].
BRICHARD, SM ;
BAILEY, CJ ;
HENQUIN, JC .
DIABETES, 1990, 39 (11) :1326-1332
[8]  
CADENE A, 1997, EUR J PHARMACOL, V318, P145
[9]  
CHUTHAPUTTI A, 1987, RES COMMUN CHEM PATH, V57, P329
[10]  
COORDT MC, 1995, P SOC EXP BIOL MED, V209, P213