Increased leptin expression in endometriosis cells is associated with endometrial stromal cell proliferation and leptin gene up-regulation

被引:73
作者
Wu, MH
Chuang, PC
Chen, HM
Lin, CC
Tsai, SJ [1 ]
机构
[1] Natl Cheng Kung Univ, Inst Clin Med, Tainan 70101, Taiwan
[2] Natl Cheng Kung Univ, Dept Obstet & Gynecol, Tainan 70101, Taiwan
[3] Natl Cheng Kung Univ, Coll Med, Dept Physiol, Tainan 70101, Taiwan
关键词
endometriosis; leptin; leptin receptor; quantitative RT-PCR; H-3]thymidine incorporation;
D O I
10.1093/molehr/8.5.456
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Endometriosis is a polygenic disease with complex, multifactorial aetiologies affecting similar to10% of women of reproductive age. Leptin is the product of the ob gene, which is related to reproductive function and immunological alteration. The angiogenic and mitogenic action of leptin may influence the formation of endometriosis. This study was aimed at determining whether leptin and leptin receptor expression differs in eutopic and ectopic endometria collected from laparoscopy and at investigating the pathophysiological role of leptin in the development of endometriosis. Leptin mRNA was undetectable in seven out of 14 eutopic endometria and only a minute amount was detected in the remaining samples. In contrast, there was a marked increase in leptin mRNA and protein expression in ectopic endometriotic lesions of patients with endometriosis (P < 0.05). Receptors for leptin were immunologically stained in eutopic endometrium as well as in ectopic endometriotic implants. However, the levels of mRNA for the long and total forms of leptin receptors were suppressed in association with the severity of endometriosis (P < 0.05). Administration of leptin stimulated its own mRNA expression in ectopic endometriotic stromal cells but decreased steady-state concentrations of mRNA encoding for leptin receptor (n = 6). In addition, leptin significantly enhanced both eutopic and ectopic endometrial stromal cell proliferation (P < 0.05). In conclusion, the differential distribution of mRNA for leptin and its receptor suggests an important autocrine and paracrine role for leptin in human endometriosis. The mitogenic and auto-augmentation effects of leptin may further contribute to the pathogenesis of endometriosis.
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收藏
页码:456 / 464
页数:9
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