Comparative analysis of polymorphism and chromosomal location of tomato microsatellite markers isolated from different sources

被引：79

作者：

Areshchenkova, T ^{[1
]}

Ganal, MW ^{[1
]}

机构：

[1] IPK, Inst Plant Genet & Crop Plant Res, D-06466 Gatersleben, Germany

来源：

THEORETICAL AND APPLIED GENETICS | 2002年 / 104卷 / 2-3期

关键词：

microsatellites; genetic mapping; Lycopersicon esculentum; genetic variability;

D O I：

10.1007/s00122-001-0775-2

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Previously isolated tomato (Lycopersicon esculentum) microsatellite markers were mainly clustered in the centromeric heterochromatin and not located in euchromatic regions. To achieve a more-uniform distribution of microsatellite markers for genome mapping purposes, a set of tomato microsatellite markers containing dinucleotide simple sequence repeats were developed by screening genomic libraries enriched for single-copy sequences, and screening the tomato EST database. The tomato microsatellites isolated in these ways were characterized by combinations of different types of repeated motifs and they were polymorphic in a set of L. esculentum varieties detecting up to four alleles. A total of 20 markers were placed on the genetic map of tomato. Interestingly, all markers isolated from genomic libraries enriched for single-copy sequences by PstI-pre-digestion mapped into the centromeric regions. The majority of markers derived from EST sequences contained predominantly AT microsatellites and were located in euchromatic regions.

引用

页码：229 / 235

页数：7

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