Sensitive in vivo imaging of T cells using a membrane-bound Gaussia princeps luciferase

被引:99
作者
Santos, Elmer B. [2 ,3 ,4 ]
Yeh, Raymond [1 ]
Lee, James [1 ]
Nikhamin, Yan [1 ]
Punzalan, Blesida [2 ,3 ]
Punzalan, Blesserene [2 ,3 ]
La Perle, Krista [5 ]
Larson, Steven M. [2 ,3 ,4 ]
Sadelain, Michel [1 ,3 ,6 ,7 ]
Brentjens, Renier J. [1 ,7 ]
机构
[1] Mem Sloan Kettering Canc Ctr, Dept Med, New York, NY 10021 USA
[2] Mem Sloan Kettering Canc Ctr, Dept Radiol, New York, NY 10021 USA
[3] Mem Sloan Kettering Canc Ctr, Mol Pharmacol & Chem Program, New York, NY 10021 USA
[4] Mem Sloan Kettering Canc Ctr, Ludwig Ctr Canc Immunotherapy, New York, NY 10021 USA
[5] Mem Sloan Kettering Canc Ctr, Res Anim Resource Ctr, New York, NY 10021 USA
[6] Mem Sloan Kettering Canc Ctr, Gene Transfer & Somat Cell Engn Lab, New York, NY 10021 USA
[7] Mem Sloan Kettering Canc Ctr, Ctr Cell Engn, New York, NY 10021 USA
基金
美国国家卫生研究院;
关键词
COLLAGEN-INDUCED ARTHRITIS; LYMPHOCYTE TRAFFICKING; ADOPTIVE IMMUNOTHERAPY; PROSTATE-CANCER; GENE-TRANSFER; EXPRESSION; BIOLUMINESCENCE; TUMORS; VISUALIZATION; REJECTION;
D O I
10.1038/nm.1930
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We developed a new approach to bioluminescent T cell imaging using a membrane-anchored form of the Gaussia luciferase (GLuc) enzyme, termed extGLuc, which we could stably express in both mouse and human primary T cells. In vitro, extGLuc(+) cells emitted significantly higher bioluminescent signal when compared to cells expressing GLuc, Renilla luciferase (RLuc) or membrane-anchored RLuc (extRLuc). In vivo, mouse extGLuc(+) T cells showed higher bioluminescent signal when compared to GLuc(+) and RLuc(+) T cells. Application of this imaging approach to human T cells genetically modified to express tumor-specific chimeric antigen receptors (CARs) enabled us to show in vivo CAR-mediated T cell accumulation in tumor, T cell persistence over time and concomitant imaging of T cells and tumor cells modified to express firefly luciferase. This sensitive imaging technology has application to many in vivo cell-based studies in a wide array of mouse models.
引用
收藏
页码:338 / 344
页数:7
相关论文
共 36 条
[1]   A Highly Sensitive Assay for Monitoring the Secretory Pathway and ER Stress [J].
Badr, Christian E. ;
Hewett, Jeffrey W. ;
Breakefield, Xandra O. ;
Tannous, Bakhos A. .
PLOS ONE, 2007, 2 (06)
[2]   In vivo analyses of early events in acute graft-versus-host disease reveal sequential infiltration of T-cell subsets [J].
Beilhack, A ;
Schulz, S ;
Baker, J ;
Beilhack, GF ;
Wieland, CB ;
Herman, EI ;
Baker, EM ;
Cao, YA ;
Contag, CH ;
Negrin, RS .
BLOOD, 2005, 106 (03) :1113-1122
[3]   T-Cell homing to the pancreas in autoimmune mouse model of diabetes: In vivo MR imaging [J].
Billotey, C ;
Aspord, C ;
Beuf, O ;
Piaggio, E ;
Gazeau, F ;
Janier, MF ;
Thivolet, C .
RADIOLOGY, 2005, 236 (02) :579-587
[4]   Genetically targeted T cells eradicate systemic acute lymphoblastic leukemia xenografts [J].
Brentjens, Renier J. ;
Santos, Elmer ;
Nikhamin, Yan ;
Yeh, Raymond ;
Matsushita, Maiko ;
La Perle, Krista ;
Quintas-Cardama, Alfonso ;
Larson, Steven M. ;
Sadelain, Michel .
CLINICAL CANCER RESEARCH, 2007, 13 (18) :5426-5435
[5]   Eradication of systemic B-cell tumors by genetically targeted human T lymphocytes co-stimulated by CD80 and interleukin-15 [J].
Brentjens, RJ ;
Latouche, JB ;
Santos, E ;
Marti, F ;
Gong, MC ;
Lyddane, C ;
King, PD ;
Larson, S ;
Weiss, M ;
Rivière, I ;
Sadelain, M .
NATURE MEDICINE, 2003, 9 (03) :279-286
[6]   Molecular imaging using labeled donor tissues reveals patterns of engraftment, rejection, and survival in transplantation [J].
Cao, YA ;
Bachmann, MH ;
Beilhack, A ;
Yang, Y ;
Tanaka, M ;
Swijnenburg, RJ ;
Reeves, R ;
Taylor-Edwards, C ;
Schulz, S ;
Doyle, TC ;
Fathman, CG ;
Robbins, RC ;
Herzenberg, LA ;
Negrin, RS ;
Contag, CH .
TRANSPLANTATION, 2005, 80 (01) :134-139
[7]   Shifting foci of hematopoiesis during reconstitution from single stem cells [J].
Cao, YA ;
Wagers, AJ ;
Beilhack, A ;
Dusich, J ;
Bachmann, MH ;
Negrin, RS ;
Weissman, IL ;
Contag, CH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (01) :221-226
[8]   An improved and secreted luciferase reporter for schistosomes [J].
Cheng, Guofeng ;
Davis, Richard E. .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2007, 155 (02) :167-171
[9]   Adoptive immunotherapy of experimental autoimmune encephalomyelitis via T cell delivery of the IL-12 p40 subunit [J].
Costa, GL ;
Sandora, MR ;
Nakajima, A ;
Nguyen, EV ;
Taylor-Edwards, C ;
Slavin, AJ ;
Contag, CH ;
Fathman, CG ;
Benson, JM .
JOURNAL OF IMMUNOLOGY, 2001, 167 (04) :2379-2387
[10]   Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging [J].
Edinger, M ;
Cao, YA ;
Verneris, MR ;
Bachmann, MH ;
Contag, CH ;
Negrin, RS .
BLOOD, 2003, 101 (02) :640-648