Reductive transformation of bound trinitrophenyl residues and free TNT during a bioremediation process analyzed by immunoassay

被引:26
作者
Achtnich, C
Pfortner, P
Weller, MG
Niessner, R
Lenke, H
Knackmuss, HJ
机构
[1] Fraunhofer Inst Grenzflachen & Bioverfahrenstech, D-70569 Stuttgart, Germany
[2] Tech Univ Munich, Inst Wasserchem, D-81377 Munich, Germany
[3] Univ Stuttgart, Inst Mikrobiol, D-70569 Stuttgart, Germany
关键词
D O I
10.1021/es9901765
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
To follow the fate of bound metabolites of TNT in soil, a synthetic trinitrophenyl residue covalently linked to humic acids was used as model compound. A selective monoclonal antibody was able to detect chemical changes of the nitro groups of the bound residues. The general possibility of reductive transformations of nitro groups of bound molecules and the reductions rates should be determined. In comparison to the reduction of free TNT a nd its metabolites, the reductive transformation of the bound trinitrophenyl residue was delayed, and the transformation rate was considerably slower. Trinitrophenyl residues also could be detected by the immunoassay in humic acids extracted from TNT contaminated soil. The reductive transformation of these trinitrophenyl residues started after the reduction of free TNT. At the end of the treatment, small amounts of these residues were still detectable indicating that some of these structures were not completely reduced during the process, From present results one can conclude that the further reduction of nitro groups of bound metabolites requires a prolonged anaerobic treatment, Not only the monitoring of free nitroaromatic compounds is recommended during the bioremediation process but also the measurement of bound residues to determine the optimal conditions acid duration of the treatment.
引用
收藏
页码:3421 / 3426
页数:6
相关论文
共 34 条
[1]  
ACHTNICH C, IN PRESS ENV TOXICOL
[2]   METABOLISM OF 2,4,6-TRINITROTOLUENE BY A PSEUDOMONAS-CONSORTIUM UNDER AEROBIC CONDITIONS [J].
BOOPATHY, R ;
MANNING, J ;
MONTEMAGNO, C ;
KULPA, C .
CURRENT MICROBIOLOGY, 1994, 28 (03) :131-137
[3]  
BOOPATHY R, 1993, APPL MICROBIOL BIOT, V39, P270, DOI 10.1007/BF00228618
[4]   BIOTRANSFORMATION OF 2,4,6-TRINITROTOLUENE (TNT) BY A METHANOCOCCUS SP (STRAIN-B) ISOLATED FROM A LAKE SEDIMENT [J].
BOOPATHY, R ;
KULPA, CF .
CANADIAN JOURNAL OF MICROBIOLOGY, 1994, 40 (04) :273-278
[5]  
BREITUNG J, 1996, UMWELTCHEM OKOTOX, V8, P249
[6]   BIODEGRADATION OF 2,4,6-TRINITROTOLUENE BY PHANEROCHAETE-CHRYSOSPORIUM - IDENTIFICATION OF INITIAL DEGRADATION PRODUCTS AND THE DISCOVERY OF A TNT METABOLITE THAT INHIBITS LIGNIN PEROXIDASES [J].
BUMPUS, JA ;
TATARKO, M .
CURRENT MICROBIOLOGY, 1994, 28 (03) :185-190
[7]   Biological treatment of TNT-contaminated soil. 1. Anaerobic cometabolic reduction and interaction of TNT and metabolites with soil components [J].
Daun, G ;
Lenke, H ;
Reuss, M ;
Knackmuss, HJ .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1998, 32 (13) :1956-1963
[8]   REDUCTION OF SUBSTITUTED NITROBENZENES IN AQUEOUS-SOLUTIONS CONTAINING NATURAL ORGANIC-MATTER [J].
DUNNIVANT, FM ;
SCHWARZENBACH, RP ;
MACALADY, DL .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1992, 26 (11) :2133-2141
[9]   Transformation of 2,4,6-trinitrotoluene by Pseudomonas pseudoalcaligenes JS']JS52 [J].
Fiorella, PD ;
Spain, JC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (05) :2007-2015
[10]   INITIAL-PHASE OPTIMIZATION FOR BIOREMEDIATION OF MUNITION COMPOUND-CONTAMINATED SOILS [J].
FUNK, SB ;
ROBERTS, DJ ;
CRAWFORD, DL ;
CRAWFORD, RL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (07) :2171-2177