Genetic Organization of the citCDEF locus and identification of mae and clyR genes from Leuconostoc mesenteroides

被引：17

作者：

Ali, SBS ^{[1
]}

Diviès, C ^{[1
]}

Prévost, H ^{[1
]}

机构：

[1] Univ Bourgogne 1, Ecole Natl Super Biol Appl Nutr & Alimentat, Microbiol Lab, UA INRA, F-21000 Dijon, France

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 14期

关键词：

D O I：

10.1128/JB.181.14.4411-4416.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this paper, we describe two open reading frames coding for a NAD-dependent malic enzyme (mae) and a putative regulatory protein (clyR) found in the upstream region of citCDEFG of Leuconostoc mesenteroides subsp. cremoris 195. The transcriptional analysis of the citrate lyase locus revealed one polycistronic mRNA covering the mae and citCDEF genes. This transcript was detected only on RNA prepared from cells grown in the presence of citrate. Primer extension experiments suggest that clyR and the citrate lyase operon are expressed from a bidirectional A-T-rich promoter region located between mae and clyR.

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页码：4411 / 4416

页数：6

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