Activation-induced expression of vascular permeability factor by human peripheral T cells: A non-radioisotopic semiquantitative reverse transcription polymerase chain reaction assay

被引:37
作者
Iijima, K
Yoshikawa, N
Nakamura, H
机构
[1] Department of Pediatrics, Kobe University School of Medicine, Kobe 650, 5-2 Kusunoki-cho 7 chome, Chuo-ku
关键词
vascular permeability factor vascular endothelial growth factor; T cell activation; reverse transcription polymerase chain reaction;
D O I
10.1016/0022-1759(96)00129-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Vascular permeability factor, also known as vascular endothelial growth factor (VPF/VEGF), is a disulfide-linked dimeric glycoprotein of about 40 kDa that enhances vascular permeability, induces chemotaxis and activation of monocytes/macrophages, and promotes growth of vascular endothelial cells. It has been reported that several tumor cell lines and normal cells produce VPF/VEGF. To examine the possibility of VPF/VEGF mRNA expression in human peripheral T cells and its mechanism(s) of regulation, we developed a non-radioisotopic semiquantitative reverse transcription-polymerase chain reaction (RT-PCR; VPF/VEGF, GAPDH co-amplification) assay which detects all species of VPF/VEGF mRNA alternative splicing products. T cells expressed negligible VPF/VEGF mRNA in the resting state. However, TPA markedly enhanced the expression of 121-, 165- and 189-amino-acid-containing isoforms of VPF/VEGF mRNA in T cells. Both CD4(+) and CD8(+) T cells expressed VPF/VEGF mRNA in response to TPA treatment. Moreover, T cell receptor stimulation with monoclonal anti-CDS antibody with or without IL-1 beta enhanced VPF/VEGF mRNA expression in T cells. These findings suggest that T cell activation induces VPF/VEGF expression in the cells, resulting in induction of its biological activities.
引用
收藏
页码:199 / 209
页数:11
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