Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos

被引：393

作者：

Fan, Xiaoying ^{[1
,2
]}

Zhang, Xiannian ^{[1
,3
]}

Wu, Xinglong ^{[1
,5
,7
]}

Guo, Hongshan ^{[1
,2
]}

Hu, Yuqiong ^{[1
,5
,7
]}

Tang, Fuchou ^{[1
,2
,4
,5
,6
]}

Huang, Yanyi ^{[1
,3
,5
]}

机构：

[1] Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, Beijing 100871, Peoples R China

[2] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China

[3] Peking Univ, Coll Engn, Beijing 100871, Peoples R China

[4] Peking Univ, Key Lab Cell Proliferat & Differentiat, Minist Educ, Beijing 100871, Peoples R China

[5] Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China

[6] Peking Univ, Hlth Sci Ctr, Ctr Mol & Translat Med, Beijing 100191, Peoples R China

[7] Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China

来源：

GENOME BIOLOGY | 2015年 / 16卷

基金：

中国国家自然科学基金;

关键词：

GENE-EXPRESSION;

D O I：

10.1186/s13059-015-0706-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Circular RNAs (circRNAs) are a new class of non-polyadenylated non-coding RNAs that may play important roles in many biological processes. Here we develop a single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq) method to sequence both polyadenylated and non-polyadenylated RNAs from individual cells. This method exhibits robust sensitivity, precision and accuracy. We discover 2891 circRNAs and 913 novel linear transcripts in mouse preimplantation embryos and further analyze the abundance of circRNAs along development, the function of enriched genes, and sequence features of circRNAs. Our work is key to deciphering regulation mechanisms of circRNAs during mammalian early embryonic development.

引用

页数：17

共 37 条

[1]

circRNA Biogenesis Competes with Pre-mRNA Splicing [J].