Methanobacterium thermoautotrophicum RNA polymerase and transcription in vitro

被引：50

作者：

Darcy, TJ

Hausner, W

Awery, DE

Edwards, AM

Thomm, M

Reeve, JN ^{[1
]}

机构：

[1] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA

[2] Univ Kiel, Inst Allgemeine Mikrobiol, D-24188 Kiel, Germany

[3] Univ Toronto, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 14期

关键词：

D O I：

10.1128/JB.181.14.4424-4429.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

RNA polymerase (RNAP) purified from Methanobacterium thermoautotrophicum Delta H has been shown to initiate transcription accurately in vitro from the hmtB archaeal histone promoter with either native or recombinant forms of the M. thermoautotrophicum TATA-binding protein and transcription factor TFB. Efforts to obtain transcription initiation from hydrogen-regulated methane gene promoters were, however, unsuccessful. Two previously unrecognized archaeal RNAP subunits have been identified, and complex formation by the M. thermoautotrophicum RNAP and TFB has been demonstrated.

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收藏

页码：4424 / 4429

页数：6

相关论文

共 29 条

[1]

BROWN JW, 1989, FEMS MICROBIOL LETT, V60, P131, DOI 10.1111/j.1574-6968.1989.tb03432.x

[2] Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii [J].

Bult, CJ ;

White, O ;

Olsen, GJ ;

Zhou, LX ;

Fleischmann, RD ;

Sutton, GG ;

Blake, JA ;

FitzGerald, LM ;

Clayton, RA ;

Gocayne, JD ;

Kerlavage, AR ;

Dougherty, BA ;

Tomb, JF ;

Adams, MD ;

Reich, CI ;

Overbeek, R ;

Kirkness, EF ;

Weinstock, KG ;

Merrick, JM ;

Glodek, A ;

Scott, JL ;

Geoghagen, NSM ;

Weidman, JF ;

Fuhrmann, JL ;

Nguyen, D ;

Utterback, TR ;

Kelley, JM ;

Peterson, JD ;

Sadow, PW ;

Hanna, MC ;

Cotton, MD ;

Roberts, KM ;

Hurst, MA ;

Kaine, BP ;

Borodovsky, M ;

Klenk, HP ;

Fraser, CM ;

Smith, HO ;

Woese, CR ;

Venter, JC .

SCIENCE, 1996, 273 (5278) :1058-1073

[3] Comparison of in-gel and on-membrane digestion methods at low to sub-pmol level for subsequent peptide and fragment-ion mass analysis using matrix-assisted laser-desorption ionization mass spectrometry [J].

Courchesne, PL ;

Luethy, R ;

Patterson, SD .

ELECTROPHORESIS, 1997, 18 (3-4) :369-381

[4]

EDWARDS AM, 1991, J BIOL CHEM, V266, P71

[5] In vitro assembly of an archaeal D-L-N RNA polymerase subunit complex reveals a eukaryote-like structural arrangement [J].

Eloranta, JJ ;

Kato, A ;

Teng, MS ;

Weinzierl, ROJ .

NUCLEIC ACIDS RESEARCH, 1998, 26 (24) :5562-5567

[6]

FITZGIBBON S, 1999, COMMUNICATION

[7] PROMOTER RECOGNITION IN ARCHAEA IS MEDIATED BY TRANSCRIPTION FACTORS - IDENTIFICATION OF TRANSCRIPTION FACTOR ATFB FROM METHANOCOCCUS-THERMOLITHOTROPHICUS AS ARCHAEAL TATA-BINDING PROTEIN [J].

GOHL, HP ;

GRONDAHL, B ;

THOMM, M .

NUCLEIC ACIDS RESEARCH, 1995, 23 (19) :3837-3841

[8] HOMOLOGIES OF COMPONENTS OF DNA-DEPENDENT RNA-POLYMERASES OF ARCHAEBACTERIA, EUKARYOTES AND EUBACTERIA [J].

GROPP, F ;

REITER, WD ;

SENTENAC, A ;

ZILLIG, W ;

SCHNABEL, R ;

THOMM, M ;

STETTER, KO .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1986, 7 (01) :95-101

[9] Two transcription factors related with the eucaryal transcription factors TATA-binding protein and transcription factor IIB direct promoter recognition by an archaeal RNA polymerase [J].

Hausner, W ;

Wettach, J ;

Hethke, C ;

Thomm, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (47) :30144-30148

[10] Transcriptional regulation: Contending with complexity [J].

Holstege, FCP ;

Young, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (01) :2-4