Site-selective protein immobilization by Staudinger ligation

被引：112

作者：

Watzke, A

Köhn, M

Gutierrez-Rodriguez, M

Wacker, R

Schröder, H

Breinbauer, R

Kuhlmann, J

Alexandrov, K

Niemeyer, CM

Goody, RS

Waldmann, H

机构：

[1] Max Planck Inst Mol Physiol, Dept Phys Biochem, D-44227 Dortmund, Germany

[2] Max Planck Inst Mol Physiol, Dept Biol Chem, D-44227 Dortmund, Germany

[3] Univ Dortmund, Fachbereich Chem, D-44227 Dortmund, Germany

[4] Max Planck Inst Mol Physiol, Dept Biol Struct, D-44227 Dortmund, Germany

[5] Chimera Biotec GmbH, D-44227 Dortmund, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 09期

关键词：

bioorganic chemistry; protein microarrays; protein modification; solid-phase synthesis; Staudinger ligation;

D O I：

10.1002/anie.200502057

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A sticky situation: A site-specific immobilization of proteins onto a glass surface may allow the creation of protein microarrays. This approach relies on the Staudinger ligation between azide-modified proteins and a phosphane-functionalized glass surface. (Figure Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1408 / 1412

页数：5

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