High Rhinovirus Burden in Lower Airways of Children With Cystic Fibrosis

被引:61
作者
Kieninger, Elisabeth [1 ,2 ]
Singer, Florian [1 ]
Tapparel, Caroline [3 ]
Alves, Marco P. [1 ,2 ]
Latzin, Philipp [1 ,2 ]
Tan, Hui-Leng [4 ]
Bossley, Cara [4 ]
Casaulta, Carmen [1 ]
Bush, Andrew [4 ]
Davies, Jane C. [4 ]
Kaiser, Laurent [3 ]
Regamey, Nicolas [1 ,2 ]
机构
[1] Univ Hosp Bern, Dept Pediat, Div Pediat Resp Med, CH-3010 Bern, Switzerland
[2] Univ Bern, Dept Clin Res, Bern, Switzerland
[3] Univ Hosp Geneva, Virol Lab, Div Infect Dis, Geneva, Switzerland
[4] Royal Brompton Hosp, Dept Pediat Resp Med, London SW3 6LY, England
基金
瑞士国家科学基金会;
关键词
RESPIRATORY VIRAL-INFECTIONS; OBSTRUCTIVE PULMONARY-DISEASE; PSEUDOMONAS-AERUGINOSA; INFLAMMATORY RESPONSE; VIRUS-INFECTIONS; YOUNG-CHILDREN; ASTHMA; TRACT; LOAD; VACCINATION;
D O I
10.1378/chest.12-0954
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Background: Rhinovirus (RV)-induced pulmonary exacerbations are common in cystic fibrosis (CF) and have been associated with impaired virus clearance by the CF airway epithelium in vitro. Here, we assess in vivo the association of RV prevalence and load with antiviral defense mechanisms, airway inflammation, and lung function parameters in children with CF compared with a control group and children with other chronic respiratory diseases. Methods: RV presence and load were measured by real-time reverse transcription-polymerase chain reaction in BAL samples and were related to antiviral and inflammatory mediators measured in BAL and to clinical parameters. Results: BAL samples were obtained from children with CF (n = 195), non-CF bronchiectasis (n = 40), or asthma (n = 29) and from a control group (n = 35) at a Median (interquartile range [IQR]) age of 8.2 (4.0-11.7) years. RV was detected in 73 samples (24.4%). RV prevalence was similar among groups. RV load (median [IQR] x 10(3) copies/mL) was higher in children with CF (143.0 [13.1-1530.0]), especially during pulmonary exacerbations, compared with children with asthma (3.0 [1.3-25.8], P = .006) and the control group (0.5 [0.3-0.5], P < .001), but similar to patients with non-CF bronchiectasis (122.1 [2.7-4423.5], P = not significant). In children with CF, RV load was negatively associated with interferon (IFN)-beta and IFN-lambda, IL-1ra levels, and FEV1, and positively with levels of the cytokines CXCL8 and CXCL10. Conclusions: RV load in CF BAL is high, especially during exacerbated lung disease. Impaired production of antiviral mediators may lead to the high RV burden in the lower airways of children with CF Whether high RV load is a cause or a consequence of inflammation needs further investigation in longitudinal studies. CHEST 2013; 143(3):782-790
引用
收藏
页码:782 / 790
页数:9
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