Population structure of recrudescent Plasmodium falciparum isolates from Western Uganda

被引:23
作者
Jelinek, T
Kilian, AHD
Westermeier, A
Pröll, S
Kabagambe, G
Nothdurft, HD
von Sonnenburg, F
Löscher, T
机构
[1] Univ Munich, Dept Infect Dis & Trop Med, D-80802 Munich, Germany
[2] GTZ, Basic Hlth Serv, Ft Portal, Uganda
关键词
malaria; P; falciparum; epidemiology; treatment; drug resistance; genotyping;
D O I
10.1046/j.1365-3156.1999.00428.x
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
It has been proposed that polymorphisms of the Merozoite Surface Protein 1 and 2 (MSPI and MSP2) and the Glutamate Rich Protein (GLURP) genes can be considered as genetic markers for the genotyping of field populations of Plasmodoium falciparum. During a field study on in vivo drug resistance against chloroquine, sulphadoxine/pyrimethamine (S/P) and cotrimoxazole in West Uganda, sensitive and resistant isolates were collected from patients by fingerprick for genotyping. 59 (72.8%) of the 81 P. falciparum samples isolated at day 0 showed multiclonal infection with 2-7 clones. Among the isolates we investigated, presence of the allelic family MAD20 of MSP1 at day 0 was significantly (P = 0.0041) associated with decreased resistance to antimalarials. Use of this method in a field study on in vivo drug resistance demonstrates another potential application of genotyping as a tool for epidemiological investigations.
引用
收藏
页码:476 / 480
页数:5
相关论文
共 22 条
  • [1] Evidence that recurrent Plasmodium falciparum infection is caused by recrudescence of resistant parasites
    AlYaman, F
    Genton, B
    Reeder, JC
    Anders, RF
    Alpers, MP
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1997, 56 (04) : 436 - 439
  • [2] ANDERS RF, 1989, BLOOD, V74, P1865
  • [3] A clonal Plasmodium falciparum population in an isolated outbreak of malaria in the Republic of Cabo Verde
    Arez, AP
    Snounou, G
    Pinto, J
    Sousa, CA
    Modiano, D
    Ribeiro, H
    Franco, AS
    Alves, J
    do Rosario, VE
    [J]. PARASITOLOGY, 1999, 118 : 347 - 355
  • [4] Population structure of Plasmodium falciparum in villages with different malaria endemicity in east Africa
    Babiker, HA
    Lines, J
    Hill, WG
    Walliker, D
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1997, 56 (02) : 141 - 147
  • [5] How does molecular epidemiology help to understand malaria?
    Beck, HP
    [J]. TROPICAL MEDICINE & INTERNATIONAL HEALTH, 1999, 4 (01) : 1 - 3
  • [6] Application of genetic markers to the identification of recrudescent Plasmodium falciparum infections on the northwestern border of Thailand
    Brockman, A
    Paul, REL
    Anderson, TJC
    Hackford, I
    Phaiphun, L
    Looareesuwan, S
    Nosten, F
    Day, KP
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1999, 60 (01) : 14 - 21
  • [7] THE EPIDEMIOLOGY OF MALARIA IN A POPULATION SURROUNDING MADANG, PAPUA-NEW-GUINEA
    CATTANI, JA
    TULLOCH, JL
    VRBOVA, H
    JOLLEY, D
    GIBSON, FD
    MOIR, JS
    HEYWOOD, PF
    ALPERS, MP
    STEVENSON, A
    CLANCY, R
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1986, 35 (01) : 3 - 15
  • [8] GENETIC DIVERSITY OF PLASMODIUM-FALCIPARUM SHOWS GEOGRAPHICAL VARIATION
    CREASEY, A
    FENTON, B
    WALKER, A
    THAITHONG, S
    OLIVEIRA, S
    MUTAMBU, S
    WALLIKER, D
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1990, 42 (05) : 403 - 413
  • [9] THE ENDEMIC NORMAL IN LYMPHATIC FILARIASIS - A STATIC CONCEPT
    DAY, KP
    [J]. PARASITOLOGY TODAY, 1991, 7 (12): : 341 - 343
  • [10] Daily dynamics of Plasmodium falciparum subpopulations in asymptomatic children in a holoendemic area
    Farnert, A
    Snounou, G
    Rooth, I
    Bjorkman, A
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1997, 56 (05) : 538 - 547