Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida

被引：51

作者：

Fairey, ER

Edmunds, JSG

Deamer-Melia, NJ

Glasgow, H

Johnson, FM

Moeller, PR

Burkholder, JM

Ramsdell, JS

机构：

[1] Natl Ocean Serv, NOAA, Ctr Coastal Environm Hlth & Biomol Res, Marine Biotoxins Program, Charleston, SC 29412 USA

[2] N Carolina State Univ, Dept Bot, Raleigh, NC 27695 USA

[3] NIEHS, Intramural Res Program, Res Triangle Pk, NC 27709 USA

[4] NIEHS, Environm Toxicol Program, Res Triangle Pk, NC 27709 USA

来源：

ENVIRONMENTAL HEALTH PERSPECTIVES | 1999年 / 107卷 / 09期

关键词：

assay; c-fos; GH(4)C(1); Pfiesteria piscicida; pituitary; toxin;

D O I：

10.2307/3434655

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Collaborative studies were performed to develop a functional assay for fish-killing activity produced by P. fiesteria piscicida. Eight cell lines were used to screen organic fractions and residual water fraction by using a 3-[4,5-dimethylthiazol-(2-4)]-diphenyltetrazolium bromide cytotoxicity assay. Diethyl ether and a residual water fraction were cytotoxic to several cell lines including rat pituitary (GH(4)C(1)) cells. Residual water as well as preextracted culture water containing P. piscicida cells induced c-fos-luciferase expressed in GH(4)C(1) cells with a rapid time course of induction and sensitive detection. The reporter gene assay detected activity in toxic isolates of P. piscicida from several North Carolina estuaries in 1997 and 1998 and may also be suitable for detecting toxic activity in human and animal serum.

引用

页码：711 / 714

页数：4

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