Generation of VV-hemorphin-7 from globin by peritoneal macrophages

被引：40

作者：

Dagouassat, N ^{[1
]}

Garreau, I ^{[1
]}

Sannier, F ^{[1
]}

Zhao, QY ^{[1
]}

Piot, JM ^{[1
]}

机构：

[1] LAB GENIE PROT & CELLULAIRE POLE SCI & TECHNOL, F-17042 LA ROCHELLE 1, FRANCE

来源：

FEBS LETTERS | 1996年 / 382卷 / 1-2期

关键词：

hemorphin; hemoglobin; macrophages; lysosomal enzymes; cathepsin D; high-performance liquid chromatography;

D O I：

10.1016/0014-5793(96)00144-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), shown the release of a bioactive peptide, VV-hemorphin-7. When a carboxyl proteinase inhibitor such as pepstatin A was added, no hemorphin was generated, Our results clearly demonstrated that VV-hemorphin-7 generation was principally due to cathepsin D. This study allowed us to hypothetize a possible pathway for in vivo hemorphins appearance from globin catabolism by macrophages.

引用

页码：37 / 42

页数：6

共 28 条

[1] HIGH-MOLECULAR-WEIGHT ASPARTIC ENDOPEPTIDASE GENERATES A CORONARO-CONSTRICTORY PEPTIDE FROM THE BETA-CHAIN OF HEMOGLOBIN
BARKHUDARYAN, N
KELLERMANN, J
GALOYAN, A
LOTTSPEICH, F
[J]. FEBS LETTERS, 1993, 329 (1-2) : 215 - 218
[2] BINDLINGMEYER BA, 1984, J CHROMATOGR, V336, P93
[3] NOVEL OPIOID-PEPTIDES DERIVED FROM HEMOGLOBIN - HEMORPHINS
BRANTL, V
GRAMSCH, C
LOTTSPEICH, F
MERTZ, R
JAEGER, KH
HERZ, A
[J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1986, 125 (02) : 309 - 310
[4] OPIOID ACTIVITIES OF BETA-CASOMORPHINS
BRANTL, V
TESCHEMACHER, H
BLASIG, J
HENSCHEN, A
LOTTSPEICH, F
[J]. LIFE SCIENCES, 1981, 28 (17) : 1903 - 1909
[5] XENOPSIN-RELATED PEPTIDE(S) ARE FORMED FROM XENOPSIN PRECURSOR BY LEUKOCYTE PROTEASE(S) AND CATHEPSIN-D
CARRAWAY, RE
MITRA, SP
MURAKI, K
[J]. PEPTIDES, 1991, 12 (01) : 107 - 112
[6] CARRAWAY RE, 1986, P INT KIN M KIN, P169
[7] GENERATION OF XENOPSIN-RELATED PEPTIDES FROM TISSUE PRECURSORS BY MEDIA CONDITIONED BY ENDOTOXIN-STIMULATED RAT PERITONEAL-MACROPHAGES
COCHRANE, DE
CARRAWAY, RE
BOUCHER, W
[J]. INFLAMMATION, 1991, 15 (05) : 381 - 390
[8] FORMATION OF HISTAMINE-RELEASING ACTIVITY FROM ALBUMIN BY MEDIUM CONDITIONED BY ENDOTOXIN-STIMULATED RAT PERITONEAL-MACROPHAGES
COCHRANE, DE
BOUCHER, W
CARRAWAY, RE
[J]. AGENTS AND ACTIONS, 1992, 35 (1-2): : 19 - 28
[9] DAGOUASSAT N, 1995, IN PRESS NEUROPEPTID
[10] PEPTIDE-FRAGMENTS DERIVED FROM THE BETA-CHAIN OF HEMOGLOBIN (HEMORPHINS) ARE CENTRALLY ACTIVE INVIVO
DAVIS, TP
GILLESPIE, TJ
PORRECA, F
[J]. PEPTIDES, 1989, 10 (04) : 747 - 751

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