A highly sensitive assay for quantitation of apolipoprotein B-48 using an antibody to human apolipoprotein B and enhanced chemiluminescence

被引：47

作者：

Smith, D

Proctor, SD

Mamo, JCL

机构：

[1] UNIV WESTERN AUSTRALIA,DEPT MED,NEDLANDS,WA 6009,AUSTRALIA

[2] UNIV WESTERN AUSTRALIA,DEPT PHYSIOL,NEDLANDS,WA 6009,AUSTRALIA

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1997年 / 34卷

关键词：

apo B-100; hypercholesterolaemia; LDL; triglyceride-rich lipoprotein;

D O I：

10.1177/000456329703400210

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We describe a method for the rapid quantification of serum apolipoprotein B-48 using a commercially available anti-apolipoprotein (apo)B antiserum and compare it to analytical sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) with coomassie blue R250 staining. The method described here eliminates the need for de-lipidation of samples and only requires a one-step overnight ultracentrifugation. Western-blotting and enhanced chemiluminescence (ECL) visualization of proteins was approximately 10 times more sensitive than coomassie staining and generally took no longer to complete than staining/ destaining of SDS-PAGE gels. The sensitivity of the antiserum/ECL technique enabled quantitation of fasting apolipoprotein B-48 which could not be resolved by SDS-PAGE and Coomassie staining.

引用

页码：185 / 189

页数：5

共 10 条

[1] APOLIPOPROTEIN B-48 IS THE PRODUCT OF A MESSENGER-RNA WITH AN ORGAN-SPECIFIC IN-FRAME STOP CODON [J].

CHEN, SH ;

HABIB, G ;

YANG, CY ;

GU, ZW ;

LEE, BR ;

WENG, SA ;

SILBERMAN, SR ;

CAI, SJ ;

DESLYPERE, JP ;

ROSSENEU, M ;

GOTTO, AM ;

LI, WH ;

CHAN, L .

SCIENCE, 1987, 238 (4825) :363-366

[2]

HERBERT PN, 1973, J BIOL CHEM, V248, P4941

[3]

KARPE F, 1994, J LIPID RES, V35, P1311

[4] POSTPRANDIAL PLASMA VITAMIN-A METABOLISM IN HUMANS - A REASSESSMENT OF THE USE OF PLASMA RETINYL ESTERS AS MARKERS FOR INTESTINALLY DERIVED CHYLOMICRONS AND THEIR REMNANTS [J].

KRASINSKI, SD ;

COHN, JS ;

RUSSELL, RM ;

SCHAEFER, EJ .

METABOLISM-CLINICAL AND EXPERIMENTAL, 1990, 39 (04) :357-365

[5]

MAMO JCL, 1994, COR ART DIS, V5, P696

[6] A NOVEL ANTISERUM SPECIFIC TO APOLIPOPROTEIN-B-48 - APPLICATION IN THE INVESTIGATION OF POSTPRANDIAL LIPIDEMIA IN HUMANS [J].

PEEL, AS ;

ZAMPELAS, A ;

WILLIAMS, CM ;

GOULD, BJ .

CLINICAL SCIENCE, 1993, 85 (05) :521-524

[7] 2-STEP 3-PHOSPHOLENE TO PHOSPHORINONE TRANSFORMATION [J].

QUIN, LD ;

KISALUS, JC .

PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS, 1985, 22 (01) :35-39

[8]

THOMPSON GR, 1990, HDB HYPERLIPIDAEMIA, P26

[9] ATHEROGENESIS - POSTPRANDIAL PHENOMENON [J].

ZILVERSMIT, DB .

CIRCULATION, 1979, 60 (03) :473-485

[10]

ZILVERSMIT DB, 1989, J LIPID RES, V30, P1639

← 1 →