Recombinant human IL-2 is cytotoxic to oligodendrocytes after in vitro self aggregation

被引:30
作者
Curatolo, L [1 ]
Valsasina, B [1 ]
Caccia, C [1 ]
Raimondi, GL [1 ]
Orsini, G [1 ]
Bianchetti, A [1 ]
机构
[1] PHARMACIA & UPJOHN INC,CNS & ONCOL RES,NERVIANO,MILANO,ITALY
关键词
in vitro cytotoxicity; molecular aggregates; rat oligodendrocytes; rhIL-2;
D O I
10.1006/cyto.1997.0228
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Interleukin 2 (IL-2) is a cytokine produced by activated T cells that plays a crucial role in the immune response and exerts multiple functions in different tissues including the nervous system. The present study was carried out to investigate the effect of recombinant human IL-2 (rhIL-2) on the survival of different glial cells type and of cortical neurons in culture. The results demonstrate that rhIL-2 is selectively cytotoxic to oligodendrocytes only if preincubated in aqueous solution (1200 U/ml) for at least two days before being added to the culture. Other glial and neuronal cells were unaffected. The cytotoxic effect mas temperature-and concentration-dependent occurring only when rhIL-2 was preincubated at 37 degrees C and mas dose-dependently neutralized by antibodies raised against IL-2 indicating that an immunorective IL-2 like compound is the active principle. Polyacrilamide gel electrophoresis under native (PAGE) and denaturing (SDS-PAGE) conditions and gel filtration analysis of the rhIL-2 incubated solution suggest that rhIL-2 is cytotoxic to oligodendrocytes only after self association into soluble high weight molecular aggregates. (C) 1997 Academic Press Limited.
引用
收藏
页码:734 / 739
页数:6
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