Novel quorum-sensing-control led genes in Erwinia carotovora subsp carotovora:: Identification of a fungal elicitor homologue in a soft-rotting bacterium

被引：66

作者：

Pemberton, CL

Whitehead, NA

Sebalhia, M

Bell, KS

Hyman, LJ

Harris, SJ

Matlin, AJ

Robson, ND

Birch, PRJ

Carr, JP

Toth, IK

Salmond, GPC

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England

[2] Univ Cambridge, Dept Plant Sci, Cambridge CB2 1QW, England

[3] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2005年 / 18卷 / 04期

关键词：

secretion; soft rot;

D O I：

10.1094/MPMI-18-0343

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seven new genes controlled by the quorum-sensing signal molecule N-(3-oxohexanoyl)-(L)-homoserine lactone (OHHL) have been identified in Erwinia carotovora subsp. carotovora. Using TnphoA as a mutagen, we enriched for mutants detective in proteins that could play a role in the interaction between E. carotovora subsp. carotovora and its plant hosts, and identified NiPEcc and its counterpart in E. carotovora subsp. atroseptica. These are members of a growing family of proteins related to Nep1 from Fusarium oxysporum which can induce necrotic responses in a variety of dicotyledonous plants. NiPEcc produced necrosis in tobacco, NiPEca affected potato stem rot, and both affected virulence in potato tubers. In E. carotovora subsp. carotovora, nip was shown to be subject to weak repression by the LuxR family regulator, EccR, and may be regulated by the negative global regulator RsmA.

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收藏

页码：343 / 353

页数：11

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