A rapid diagnostic test for plague detects Yersinia pestis F1 antigen in ancient human remains.

被引：19

作者：

Bianucci, Raffaella

Rahalison, Lila

Ferroglio, Ezio

Massa, Emma Rabino

Signoli, Michel

机构：

[1] Univ Turin, Dipartimento Biol Anim & Uomo, Lab Antropol, I-10123 Turin, Italy

[2] Inst Pasteur Madagascar, Ctr Collaborateur OMS, Lab Cent Peste, Antananarivo, Madagascar

[3] Univ Turin, Dipartimento Prod Anim Epidemiol & Ecol, Settore Parassitol & Malattie Parassitarie, I-10095 Grugliasco, TO, Italy

[4] Univ Aix Marseille 2, Fac Med Marseille, CNRS, UMR 6578, F-13385 Marseille, France

[5] Univ Nice Sophia Antipolis, CNRS, UMR 6130, F-06560 Valbonne, France

来源：

COMPTES RENDUS BIOLOGIES | 2007年 / 330卷 / 10期

关键词：

RDT plague; Yersinia pestis; putative plague mass graves;

D O I：

10.1016/j.crvi.2007.07.007

中图分类号：

Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

A rapid diagnostic dipstick test (RDT) that detects Yersinia pestis F1 antigen has been recently applied on 18 putative plague victims exhumed from four archaeological burial sites in southeastern France dating back to the 16th, 17th and 18th centuries. The Y pestis antigen F1 was detected in 12 ancient samples out of 18 (67%). Negative controls confirmed their negativity (100%). Our results emphasize that the detection threshold of the RDT for plague (0.5 ng/ml) is sufficient for a first retrospective diagnosis of Y. pestis infection in ancient remains, and confirm the high specificity and sensitivity of the assay. Double-blind analyses performed by using two different techniques (RDT and 'suicide PCR') led us to the identification of the Y. pestis Fl antigen and the Y. pestis pla and gplD genes. These data provide clear evidence of the presence of Y. pestis in the examined specimens.

引用

页码：747 / 754

页数：8

共 28 条

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