Immunofluorescence method for detection of Ebola virus immunoglobulin G, using HeLa cells which express recombinant nucleoprotein

被引：15

作者：

Saijo, M ^{[1
]}

Niikura, M ^{[1
]}

Morikawa, S ^{[1
]}

Kurane, I ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Virol 1, Special Pathogens Lab, Tokyo 2080011, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 02期

关键词：

D O I：

10.1128/JCM.39.2.776-778.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleoprotein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expressed EBO NP, and this was used as an immunofluorescent (IF) antigen to detect EBO immunoglobulin G (IgG) antibody. This IF method has high efficacy in detecting EBO IgG antibody in clinical specimens, indicating its usefulness in the diagnosis of EBO infections and seroepidemiological studies.

引用

页码：776 / 778

页数：3

共 20 条

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