HPLC analysis of carbohydrate deficient transferrin isoforms isolated by the Axis-Shield %CDT method

被引：33

作者：

Aldén, A

Ohlson, S

Påhlsson, P

Rydén, I

机构：

[1] Kalmar Cty Hosp, Dept Clin Chem, Kalmar, Sweden

[2] Univ Kalmar, Dept Chem & Biomed Sci, SE-39182 Kalmar, Sweden

[3] Linkoping Univ, Dept Biomed & Surg, SE-58185 Linkoping, Sweden

来源：

CLINICA CHIMICA ACTA | 2005年 / 356卷 / 1-2期

关键词：

carbohydrate-deficient transferrin; ion-exchange chromatography; affinity chromatography; HPLC;

D O I：

10.1016/j.cccn.2005.01.021

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 [基础医学];

摘要：

Background: Carbohydrate-deficient transferrin (CDT) is elevated during prolonged overconsumption of alcohol and CDT is considered to be the most specific biochemical marker for alcohol overconsumption. However, an accurate method for analysing CDT is necessary because the test is frequently used for example in legal matters. Methods: Patient serum samples were analysed with the Axis-Shield %CDT and eluates were pooled together. Transferrin was purified from the pool by affinity chromatography and further analysed with HPLC to determine the ratios of different transferrin isoforms. Results: In the eluates using the Axis-Shield %CDT method, a substantial amount of trisialo transferrin was found, which is generally not considered a CDT isoform. Conclusions: The fact that trisialo transferrin is present may generate falsely elevated CDT results and it could at least partly explain the discrepancy between results of the Axis-Shield %CDT assay and HPLC in routine analysis. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：143 / 146

页数：4

共 11 条

[1]

Forensic analysis of carbohydrate-deficient transferrin (CDT): implementation of a screening and confirmatory analysis concept is hampered by the lack of CDT isoform standards [J].