Stoichiometry in zinc ion transfer from metallothionein to zinc finger peptides

被引：55

作者：

Hathout, Y

Fabris, D

Fenselau, C

机构：

[1] Univ Maryland Baltimore Cty, Dept Chem, Baltimore, MD 21250 USA

[2] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2001年 / 204卷 / 1-3期

基金：

美国国家卫生研究院;

关键词：

metal ion flux; reaction stoichiometry; zinc finger peptides; metallothionein; noncovalent complexes;

D O I：

10.1016/S1387-3806(00)00343-2

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

Electrospray and nanospray ionizations are used to study the transfer of zinc ions between Zn-7-metallothionein and apo peptides that are models for several kinds of zinc finger proteins. A membrane experiment is reported here which demonstrates that interprotein contact is required for these transfers. Analysis on a quadrupole ion trap has allowed all reactants and all products to be monitored simultaneously. Evidence is provided for the preferential transfer of a single zinc ion from each Zn-7-metallothionein complex. (C) 2001 Elsevier Science B.V.

引用

页码：1 / 6

页数：6

共 13 条

[1] CADMIUM BINDING AND METAL CLUSTER FORMATION IN METALLOTHIONEIN - A DIFFERENTIAL MODIFICATION STUDY [J].