Protection of mice with a tuberculosis subunit vaccine based on a fusion protein of antigen 85B and ESAT-6

被引:244
作者
Olsen, AW [1 ]
van Pinxteren, LAH [1 ]
Okkels, LM [1 ]
Rasmussen, PB [1 ]
Andersen, P [1 ]
机构
[1] Statens Serum Inst, Dept TB Immunol, DK-2300 Copenhagen S, Denmark
关键词
D O I
10.1128/IAI.69.5.2773-2778.2001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In this study, we investigated the potential of a tuberculosis subunit vaccine based an fusion proteins of the immunodominant antigens ESAT-6 and antigen 85B. When the fusion proteins were administered to mice in the adjuvant combination dimethyl dioctadecylammonium bromide-monophosphoryl lipid A, a strong dose-dependent immune response was induced to both single components as well as to the fusion proteins. The immune response Induced was accompanied by high levels of protective immunity and reached the level of;Mycobacterium bovis BCG-induced protection over a broad dose range. The vaccine induced efficient immunological memory, which remained stable 30 weeks postvaccination.
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页码:2773 / 2778
页数:6
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